Rabbit CD21 Polyclonal Antibody | anti-CR2 antibody

Anti-CD21 Antibody, Rabbit Polyclonal

Cat. Num. AAA27739

• Gene Names: CR2; CR; C3DR; CD21; CVID7; SLEB9
• Reactivity: Human, Cynomolgus
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CD21; Polyclonal Antibody; Anti-CD21 Antibody; Rabbit Polyclonal; CD21 Antibody; Rabbit PAb; Antigen Affinity Purified; complement component (3d/Epstein Barr virus) receptor 2; Anti-C3DR Antibody; Anti-CR Antibody; Anti-CVID7 Antibody; Anti-SLEB9 Antibody; anti-CR2 antibody

• Host: Rabbit
• Reactivity: Human, Cynomolgus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CD21
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CR2 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:25,000-1:50,000; IHC-P: 1:200-1:10,000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Human CD21 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD21 (rh CD21). CD21 specific IgG was purified by Human CD21 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IHC (Immunohistchemistry)

(Immunochemical staining CD21 in cynomolgus lymph node with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry)

(Immunochemical staining CD21 in cynomolgus spleen with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-CD21 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Membrane LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:113 kDaObserved band size:170 kDa)

IP (Immunoprecipitation)

(CD21 was immunoprecipitated using:Lane A:0.5 mg Daudi Whole Cell Lysate0.5 uL anti-CD21 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD21 rabbit polyclonal antibody,at 1:350 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 170 kDaObserved band size: 170 kDa)

IHC (Immunohistochemistry)

(Immunochemical staining CD21 in human lymph node with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to germinal center. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein.)

IHC (Immunohistochemistry)

(Immunochemical staining CD21 in human tonsil with rabbit polyclonal antibody at 1:5000 dilution,formalin-fixed paraffin embedded sections. Positive staining was localized to germinal center.)

Related Product Information for anti-CR2 antibody

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD21, also known as Complement component (3d/Epstein Barr virus) receptor 2 and CR2, is a member of the CD system and is a protein involved in complement system. CD21 is present on all mature B-cells and some T-cells and follicular dendritic cells. CD21 on mature B-cells form a complex called the B cell receptor complex with two other membrane proteins, CD19 and CD81. CD21 has a function in the complement system through serving as the cellular receptor specific for ligands such as C3 and C4 which can be attached to foreign macromolecules in order to remove or uptake them. This results in B-cells having enhanced response to the antigen.

Product Categories/Family for anti-CR2 antibody

Serine Proteases and Regulators; Complement Activation Pathways

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.Joseph M, et al. (1989) Structure and Function of the Complement Receptors, CR1 (CD35) and CR2 (CD21). Advanced in immunology. 46: 183-219.Aubry JP, et al. (1992) CD21 is a ligand for CD23 and regulates IgE production. Nature 358: 505-7.

NCBI and Uniprot Product Information

• NCBI GI #: 215273962
• NCBI GeneID: 1380
• UniProt Accession #: P20023
• NCBI Official Full Name: Complement receptor type 2
• NCBI Official Synonym Full Names: complement C3d receptor 2
• NCBI Official Symbol: CR2
• NCBI Official Synonym Symbols: CR; C3DR; CD21; CVID7; SLEB9
• NCBI Protein Information: complement receptor type 2
• UniProt Protein Name: Complement receptor type 2
• UniProt Gene Name: CR2
• UniProt Synonym Gene Names: C3DR; Cr2; EBV receptor
• UniProt Entry Name: CR2_HUMAN