WB (Western Blot)
(WB: The cell lysate derived from Jurkat cells was immunoblotted by the Rabbit anti CD25 (Cat#AAA71305) @ 1:500. An immunoreactive band around ~3)
Rabbit anti-Human CD25 Polyclonal Antibody | anti-CD25 antibody
Rabbit anti CD25 Polyclonal Antibody
Gene Names
IL2RA; p55; CD25; IL2R; IMD41; TCGFR; IDDM10
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA
Purity
Affinity Purified
Synonyms
CD25, Antibody; Rabbit anti CD25 Polyclonal Antibody; IL2RA; Interleukin2 receptor alpha; anti-CD25 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody reacts with human. The other species are not tested.
Purity/Purification
Affinity Purified
Form/Format
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Sequence Length
160
Applicable Applications for anti-CD25 antibody
WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA
Preparation
Recombinant full length (1-272 aa) of human CD25.
Positive Control
Jurkat Cells, Tonsil
Cellular Location
Cell Membrane
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C - 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
WB (Western Blot)
(WB: The cell lysate derived from Jurkat cells was immunoblotted by the Rabbit anti CD25 (Cat#AAA71305) @ 1:500. An immunoreactive band around ~3)
WB (Western Blot)
(WB: The cell lysate derived from Jurkat cells was immunoblotted by the Rabbit anti CD25 (Cat#AAA71305) @ 1:500. An immunoreactive band around ~3)
Related Product Information for anti-CD25 antibody
The IL2 receptor (IL2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220 low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220 low TdT+ sIg- pro-B/pre B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T cell development in fetal and adult thymus. CD25 expression is a common denominator of a specific immunomodulatory B-cell subset ready to proliferate upon IL-2 stimulation.
References
Amu, S. and Brisslert, M. Phenotype and function of CD25-expressing B lymphocytes isolated from human umbilical cord blood. Clin. Dev. Immunol. 2011, 481948 (2011).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31 kDa
NCBI Official Full Name
CD25
NCBI Official Synonym Full Names
interleukin 2 receptor subunit alpha
NCBI Official Symbol
IL2RA
NCBI Official Synonym Symbols
p55; CD25; IL2R; IMD41; TCGFR; IDDM10
NCBI Protein Information
interleukin-2 receptor subunit alpha
UniProt Protein Name
Peripheral myelin protein 22
UniProt Gene Name
Pmp22
UniProt Synonym Gene Names
Cd25; Pmp-22; PMP-22; SAG
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Product Notes
The CD25 pmp22 (Catalog #AAA71305) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti CD25 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD25 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CD25 pmp22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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