IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD28 Antibody (C-term) with Hela cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit CD28 Polyclonal Antibody | anti-CD28 antibody
CD28 Antibody (C-term)
Gene Names
CD28; Tp44
Reactivity
Human (Predicted Reactivity: Rabbit)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CD28, Antibody; CD28 Antibody (C-term); T-cell-specific surface glycoprotein CD28; TP44; CD28; anti-CD28 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Rabbit)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CD28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-208 amino acids from the C-terminal region of human CD28.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
182-208
Sequence Length
234
Applicable Applications for anti-CD28 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD28 Antibody (C-term) with Hela cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of CD28 Antibody (C-term) with Hela cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of CD28 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). CD28 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CD28 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). CD28 (arrow) was detected using the purified Pab.)
Related Product Information for anti-CD28 antibody
CD28 costimulation is essential for CD4-positive T-cell proliferation, survival, interleukin-2 production, and T-helper type-2 (Th2) development.
Product Categories/Family for anti-CD28 antibody
References
Zocchi,M.R., et.al., Eur. J. Cancer 28A (4-5), 749-754 (1992)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25066
NCBI Official Full Name
T-cell-specific surface glycoprotein CD28 isoform 2
NCBI Official Synonym Full Names
CD28 molecule
NCBI Official Symbol
CD28
NCBI Official Synonym Symbols
Tp44
NCBI Protein Information
T-cell-specific surface glycoprotein CD28
UniProt Protein Name
T-cell-specific surface glycoprotein CD28
UniProt Gene Name
CD28
UniProt Entry Name
CD28_HUMAN
Similar Products
Product Notes
The CD28 cd28 (Catalog #AAA286649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 182-208. The CD28 Antibody (C-term) reacts with Human (Predicted Reactivity: Rabbit) and may cross-react with other species as described in the data sheet. AAA Biotech's CD28 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD28 cd28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD28, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.