Application Data
(Fig: Western blot analysis on Jurkat cell lysates using anti- CD3 zeta polyclonal antibody)
CD3 zeta Polyclonal Antibody | anti-CD3 zeta antibody
CD3 zeta Antibody
Gene Names
CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
Reactivity
Mouse, Human, Rat
Applications
Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified
Synonyms
CD3 zeta, Antibody; CD3 zeta Antibody; anti-CD3 zeta antibody
Reactivity
Mouse, Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of C-terminal residues of CD3 zeta.
Purity/Purification
Immunogen affinity purified
Form/Format
Liquid
Concentration
0.49 mg/ml (varies by lot)
Sequence Length
163
Applicable Applications for anti-CD3 zeta antibody
ICC (Immunocytochemistry), WB (Western Blot)
Preservative
0.05% Sodium Azide.
Cellular Localization
Cell Membrane
Positive Control
Jurkat
Storage Buffer
1*TBS (pH7.4), 0.5%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.
Application Data
(Fig: Western blot analysis on Jurkat cell lysates using anti- CD3 zeta polyclonal antibody)
Application Data
(Fig: Western blot analysis on Jurkat cell lysates using anti- CD3 zeta polyclonal antibody)
Related Product Information for anti-CD3 zeta antibody
CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Loss of the zeta chain results in the synthesis of unstable TCRs.
References
1. Weissman A.M., Hou D., Orloff D.G., Modi W.S., Seuanez H., O'Brien S.J., Klausner R.D.; "Molecular cloning and chromosomal localization of the human T-cell receptor zeta chain: distinction from the molecular CD3.complex."; Proc. Natl. Acad. Sci. U.S.A. 85:9709-971.(1.88). 2. Rieux-Laucat F., Hivroz C., Lim A., Mateo V., Pellier I., Selz F., Fischer A., Le Deist F.; "Inherited and somatic CD3.eta mutations in a patient with T-cell deficiency."; N. Engl. J. Med. 3.4:1.1.-1.2.(2.06).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
16kDa
NCBI Official Full Name
T-cell surface glycoprotein CD3 zeta chain isoform 2
NCBI Official Synonym Full Names
CD247 molecule
NCBI Official Symbol
CD247
NCBI Official Synonym Symbols
T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
NCBI Protein Information
T-cell surface glycoprotein CD3 zeta chain
UniProt Protein Name
T-cell surface glycoprotein CD3 zeta chain
UniProt Gene Name
CD247
UniProt Synonym Gene Names
CD3Z; T3Z; TCRZ
Similar Products
Product Notes
The CD3 zeta cd247 (Catalog #AAA111244) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CD3 zeta Antibody reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 zeta can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD3 zeta cd247 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3 zeta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.