Rabbit CD36 Polyclonal Antibody | anti-CD36 antibody

Rabbit anti-Human, Mouse CD36 Polyclonal Antibody

Cat. Num. AAA289822

• Gene Names: CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• Reactivity: Reactivity: Human, Mouse; Predicted: Rat
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot, Immunofluorescence
• Synonyms: CD36, Antibody; Rabbit anti-Human, Mouse CD36 Polyclonal Antibody; Platelet glycoprotein 4, Fatty acidtranslocase, FAT, Glycoprotein IIIb, GPIIIB, Leukocyte differentiation antigen CD36, PAS IV, PAS-4, Platelet collagen receptor, Platelet glycoprotein IV, GPIV, Thrombospondin receptor, CD36, CD36, GP3B, GP4; anti-CD36 antibody

• Host: Rabbit
• Reactivity: Reactivity: Human, Mouse; Predicted: Rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Applicable Applications for anti-CD36 antibody: ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence)
• Function: Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptorligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption (By similarity) (PubMed: , PubMed: ). In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway (By similarity) (PubMed: ). Involved in oral fat perception and preferences (PubMed: , PubMed: ). Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions (By similarity). In taste receptor cells, mediates the induction of an increase in intracellular calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract (By similarity). Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis (By similarity). Receptor for thombospondins, THBS1 and THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome (By similarity) (PubMed: ). Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (By similarity) (PubMed: ).
• Cellular Location: Cell membrane; Multi-pass membrane protein. Membrane raft. Golgi apparatus. Apical cell membrane {ECO:0000250|UniProtKB:Q08857}. Note=Upon ligand-binding, internalized through dynamin-dependent endocytosis.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of CD36 Antibody (Center) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of CEM cells using CD36 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD36 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of CD36 antibody (Center) in CEM cell line lysates (35ug/lane). CD36 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-CD36 antibody

Cancer; Cardiovascular; Immunology; Metabolism; Microbiology; Signal Transduction

References

Fry,A.E., Hum. Mol. Genet. 18 (14), 2683-2692 (2009) Chilongola,J., Trop. Med. Int. Health 14 (7), 810-816 (2009)

NCBI and Uniprot Product Information

• NCBI GI #: 48375178
• NCBI GeneID: 948
• NCBI Accession #: NP_000063.2; NP_001001547.1; NP_001001548.1; NP_001120915.1; NP_001120916.1
• NCBI GenBank Nucleotide #: NM_000072.3
• UniProt Accession #: P16671; Q13966; Q16093; Q8TCV7; Q9BPZ8; Q9BQC2; Q9BZM8; Q9BZN3; D9IX66; D9IX67; D9IX68; D9IX69
• NCBI Official Full Name: platelet glycoprotein 4 isoform 1
• NCBI Official Synonym Full Names: CD36 molecule (thrombospondin receptor)
• NCBI Official Symbol: CD36
• NCBI Official Synonym Symbols: FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
• NCBI Protein Information: platelet glycoprotein 4
• UniProt Protein Name: Platelet glycoprotein 4
• UniProt Gene Name: CD36
• UniProt Synonym Gene Names: GP3B; GP4; FAT; GPIIIB; GPIV
• UniProt Entry Name: CD36_HUMAN