Rat CD36/SR-B3 ELISA Kit | CD36 elisa kit

Rat CD36/SR-B3 PicoKine ELISA Kit

Cat. Num. AAA45870

• Gene Names: Cd36; Fat
• Reactivity: Rat
• Synonyms: CD36/SR-B3; N/A; Rat CD36/SR-B3 PicoKine ELISA Kit; Platelet glycoprotein 4; CD36 molecule (thrombospondin receptor); CD36 antigen; CD36 antigen (collagen type I receptor thrombospondin receptor); CD36 molecule; CHDS7; Cluster determinant 36; FAT; Fatty acid translocase; Glycoprotein IIIb; GP3B; GP4; GPIIIB; GPIV; Leukocyte differentiation antigen; CD36; PAS 4 protein; PAS IV; PASIV; Platelet collagen receptor; Platelet glycoprotein IV; SCARB3; Scavenger receptor class B member 3; Thrombospondin receptor; CD36 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat CD36
• Sequence Length: 472
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 218pg/ml-14,000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Rat CD36/SR-B3 PicoKine ELISA Kit standard curve)

Related Product Information for CD36 elisa kit

Principle of the assay: rat CD36 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD36 has been precoated onto 96-well plates. Standards(CHO, G30-K439) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD36 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD36 amount of sample captured in plate.
Background: CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.

NCBI and Uniprot Product Information

• NCBI GI #: 146345388
• NCBI GeneID: 29184
• UniProt Accession #: Q07969; Q925W0
• Molecular Weight: 52,731 Da
• NCBI Official Full Name: Platelet glycoprotein 4
• NCBI Official Synonym Full Names: CD36 molecule (thrombospondin receptor)
• NCBI Official Symbol: Cd36
• NCBI Official Synonym Symbols: Fat
• NCBI Protein Information: platelet glycoprotein 4; GPIV; PAS-4; GPIIIB; PAS IV; glycoprotein IIIb; fatty acid translocase; platelet glycoprotein IV; adipocyte membrane protein; fatty acid transport protein; collagen type I receptor thrombospondin receptor; CD36 antigen (collagen type I receptor, thrombospondin receptor)
• UniProt Protein Name: Platelet glycoprotein 4
• UniProt Gene Name: Cd36
• UniProt Synonym Gene Names: Fat; GPIIIB; GPIV
• UniProt Entry Name: CD36_RAT