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product-image-AAA31179_IHC8.jpg IHC (Immunohistochemistry-Paraffin) (AAA31179 at 1/100 staining human breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

Rabbit CD36 Polyclonal Antibody | anti-CD36 antibody

CD36 Antibody

Gene Names
CD36; FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
CD36, Antibody; CD36 Antibody; Adipocyte membrane protein; BDPLT10; CD36; CD36 antigen (collagen type I receptor; thrombospondin receptor); CD36 antigen; CD36 molecule (thrombospondin receptor); CD36 molecule; CD36_HUMAN; CHDS7; Cluster determinant 36; Collagen receptor; platelet; FAT; Fatty acid translocase; Fatty acid transport protein; Glycoprotein IIIb; GP IIIb; GP3B; GP4; GPIIIB; GPIV; Leukocyte differentiation antigen CD36; MGC108510; MGC91634; PAS 4 protein; PAS IV; PAS-4; PASIV; Platelet collagen receptor; Platelet glycoprotein 4; Platelet glycoprotein IV; scarb3; Scavenger receptor class B member 3; Thrombospondin receptor; anti-CD36 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CD36 Antibody detects endogenous levels of total CD36.
Purity/Purification
Peptide affinity purification
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
472
Applicable Applications for anti-CD36 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
The optimal dilution should be determined by the end user.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human CD36
Fragment
Fab fragment
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemistry-Paraffin)

(AAA31179 at 1/100 staining human breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

product-image-AAA31179_IHC8.jpg IHC (Immunohistochemistry-Paraffin) (AAA31179 at 1/100 staining human breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31179 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31179_IHC7.jpg IHC (Immunohistochemistry-Paraffin) (AAA31179 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry-Paraffin)

(AAA31179 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31179_IHC6.jpg IHC (Immunohistchemistry-Paraffin) (AAA31179 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31179 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31179_IHC5.jpg IHC (Immunohistochemistry-Paraffin) (AAA31179 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31179 at 1/100 staining Rat pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31179_IHC4.jpg IHC (Immunohistochemistry-Paraffin) (AAA31179 at 1/100 staining Rat pancreatic tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry-Paraffin)

(AAA31179 at 1/100 staining Human liver cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

product-image-AAA31179_IHC3.jpg IHC (Immunohistochemistry-Paraffin) (AAA31179 at 1/100 staining Human liver cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis on Mouse Heart tissues lysates using CD36 Antibody)

product-image-AAA31179_WB2.jpg WB (Western Blot) (Western blot analysis on Mouse Heart tissues lysates using CD36 Antibody)

WB (Western Blot)

(Western blot analysis of extracts from MCF7, using CD36 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 60 kDa.)

product-image-AAA31179_WB.jpg WB (Western Blot) (Western blot analysis of extracts from MCF7, using CD36 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 60 kDa.)
Related Product Information for anti-CD36 antibody
Function: Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
948
UniProt Accession #
Molecular Weight
53kDa, 88 kD, 60~100kDa
NCBI Official Full Name
Platelet glycoprotein 4
NCBI Official Synonym Full Names
CD36 molecule
NCBI Official Symbol
CD36
NCBI Official Synonym Symbols
FAT; GP4; GP3B; GPIV; CHDS7; PASIV; SCARB3; BDPLT10
NCBI Protein Information
platelet glycoprotein 4
UniProt Protein Name
Platelet glycoprotein 4
UniProt Gene Name
CD36
UniProt Synonym Gene Names
GP3B; GP4; FAT; GPIIIB; GPIV
UniProt Entry Name
CD36_HUMAN

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Product Notes

The CD36 cd36 (Catalog #AAA31179) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD36 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD36 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000 The optimal dilution should be determined by the end user. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. . Researchers should empirically determine the suitability of the CD36 cd36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD36, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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