IP (Immunoprecipitation)
(CD40 was immunoprecipitated using: Lane A:0.5 mg A-431 Whole Cell Lysate 4 uL anti-CD40 rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CD40 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa Observed band size :45 kDa)
Rabbit anti-Human CD40/TNFRSF5 Polyclonal Antibody | anti-CD40 antibody
Anti-CD40/TNFRSF5 Antibody, Rabbit Polyclonal
Gene Names
CD40; p50; Bp50; CDW40; TNFRSF5
Reactivity
Human
Applications
Immunoprecipitation, Immunocytochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
CD40/TNFRSF5, Antibody; Anti-CD40/TNFRSF5 Antibody, Rabbit Polyclonal; CD40/TNFRSF5 Antibody, Rabbit PAb, Antigen Affinity Purified; CD40 molecule, TNF receptor superfamily member 5; Anti-Bp50 Antibody; Anti-CDW40 Antibody; Anti-p50 Antibody; Anti-TNFRSF5 Antibody; anti-CD40 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human CD40/TNFRSF5
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD40 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human CD40/TNFRSF5 Protein ( NP_001241.1; Met1-Ala194). CD40/TNFRSF5 specific IgG was purified by Human CD40/TNFRSF5 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
IP (Immunoprecipitation)
(CD40 was immunoprecipitated using: Lane A:0.5 mg A-431 Whole Cell Lysate 4 uL anti-CD40 rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CD40 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa Observed band size :45 kDa)
IP (Immunoprecipitation)
(CD40 was immunoprecipitated using: Lane A:0.5 mg A-431 Whole Cell Lysate 4 uL anti-CD40 rabbit polyclonal antibody and 60ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-CD40 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 45 kDa Observed band size :45 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of CD40-His in HepG2 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human CD40-His polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and Cell membrane.)
IF (Immunofluorescence)
(Immunofluorescence staining of CD40-His in HepG2 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human CD40-His polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and Cell membrane.)
WB (Western Blot)
(Anti-CD40 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:42 kDa)
WB (Western Blot)
(Anti-CD40 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLysates/proteins at 30ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:42 kDa)
Related Product Information for anti-CD40 antibody
CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
Product Categories/Family for anti-CD40 antibody
References
va. Koote. C. e. al. (2000). CD40-CD4. ligand. . Leuko. Biol. 6. (1). 2-17.Bhusha. A. e. al. (2002). CD40:CD40. interaction. i. X-linke. an. non-X-linke. hyper-Ig. syndromes. Immuno. Res. 2. (3). 311-24.Chatzigeorgio. A. e. al. (2009. CD40/CD40. signalin. an. it. implicatio. i. healt. an. disease. Biofactors. 35(6). 474-83.L. R. e. al. (2009. Expressio. o. CD4. an. CD40. i. Gastri. Cance. Tissu. an. It. Clinica. Significance. In. . Mo. Sci. 10(9). 3900-17.Lieven. D. e. al. (2009. Th. multi-functionalit. o. CD40. an. it. recepto. CD4. i. atherosclerosis. Throm. Haemost. 102(2). 206-14.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,619 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 5 isoform 1
NCBI Official Synonym Full Names
CD40 molecule, TNF receptor superfamily member 5
NCBI Official Symbol
CD40
NCBI Official Synonym Symbols
p50; Bp50; CDW40; TNFRSF5
NCBI Protein Information
tumor necrosis factor receptor superfamily member 5; CD40L receptor; CD40 type II isoform; B cell-associated molecule; B cell surface antigen CD40; B-cell surface antigen CD40; CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor recept
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 5
UniProt Gene Name
CD40
UniProt Synonym Gene Names
TNFRSF5
UniProt Entry Name
TNR5_HUMAN
Similar Products
Product Notes
The CD40 cd40 (Catalog #AAA259415) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD40/TNFRSF5 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD40/TNFRSF5 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD40 cd40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD40/TNFRSF5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.