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product-image-AAA282420_IF13.jpg IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using CD44 Rabbit pAb (AAA282420) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.)

Rabbit CD44 Polyclonal Antibody | anti-CD44 antibody

CD44 Rabbit pAb

Gene Names
Cd44; Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity purification
Synonyms
CD44, Antibody; CD44 Rabbit pAb; Ly-24; Pgp-1; HERMES; anti-CD44 antibody
Ordering
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Applicable Applications for anti-CD44 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Positive Samples
Mouse lung, Mouse spleen, Rat lung
Immunogen
Recombinant protein of mouse CD44.
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Ship: Ice Pack

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using CD44 Rabbit pAb (AAA282420) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.)

product-image-AAA282420_IF13.jpg IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using CD44 Rabbit pAb (AAA282420) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of various lysates, using CD44 antibody (AAA282420) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.)

product-image-AAA282420_WB15.jpg WB (Western Blot) (Western blot analysis of various lysates, using CD44 antibody (AAA282420) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.)
Related Product Information for anti-CD44 antibody
Enables hyaluronic acid binding activity and type II transforming growth factor beta receptor binding activity. Contributes to cytokine binding activity and cytokine receptor activity. Involved in several processes, including negative regulation of T cell activation; positive regulation of protein phosphorylation; and regulation of intracellular signal transduction. Acts upstream of or within several processes, including Wnt signaling pathway; morphogenesis of a branching epithelium; and wound healing involved in inflammatory response. Located in basolateral plasma membrane; external side of plasma membrane; and microvillus. Part of macrophage migration inhibitory factor receptor complex. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; genitourinary system; and limb. Human ortholog(s) of this gene implicated in breast carcinoma (multiple); carcinoma (multiple); and prostate cancer. Orthologous to human CD44 (CD44 molecule (Indian blood group)).
Product Categories/Family for anti-CD44 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,999 Da
NCBI Official Full Name
CD44 antigen isoform c
NCBI Official Synonym Full Names
CD44 antigen
NCBI Official Symbol
Cd44
NCBI Official Synonym Symbols
Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109
NCBI Protein Information
CD44 antigen
UniProt Protein Name
CD44 antigen
UniProt Gene Name
Cd44
UniProt Synonym Gene Names
Ly-24; ECMR-III; Ly-24; PGP-1; PGP-I
UniProt Entry Name
CD44_MOUSE

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Product Notes

The CD44 cd44 (Catalog #AAA282420) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD44 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD44 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the CD44 cd44 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD44, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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