WB (Western Blot)
(Applications: Western BlotSample: Tumor cell lysate and recombinant proteinDetection: SuperSignal West Pico Chemiluminescent Substrate"The rabbit antibody AAA46303 from produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting.")
anti-Human CD44 Polyclonal Antibody | anti-CD44 antibody
Anti-CD44 Antibody
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
CD44, Antibody; Anti-CD44 Antibody; CD44 antigen; LHR; BA-1; CD 44; CD44; CD44 isoform RC; CD44 molecule (Indian blood group); CD44 molecule; CD44_HUMAN; CDw44; Cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECM III; ECMR III; ECMR-III; ECMRIII; Epican; Extracellular Matrix Receptor III; GP90 Lymphocyte Homing Adhesion Receptor; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan Sulfate Proteoglycan; Hermes Antigen; homing function and Indian blood group system; HSA; HUTCH I; HUTCH-I; HUTCH1; Hyaluronate Receptor; IN; Indian blood group; Inlu Related p80 Glycoprotein; lhr; Ly 24; MC56; MDU2; MDU3; MGC10468; MIC4; MUTCH I; MUTCH1; PGP 1; PGP-1; PGP-I; PGP1; Phagocytic Glycoprotein 1; Phagocytic glycoprotein I; Transmembrane glycoprotein; anti-CD44 antibody
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
742
Applicable Applications for anti-CD44 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CD44(29-56aa RFAGVFHVEKNGRYSISRTEAADLCKAF), different from the related mouse and rat sequences by two amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Applications: Western BlotSample: Tumor cell lysate and recombinant proteinDetection: SuperSignal West Pico Chemiluminescent Substrate"The rabbit antibody AAA46303 from produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting.")
WB (Western Blot)
(Applications: Western BlotSample: Tumor cell lysate and recombinant proteinDetection: SuperSignal West Pico Chemiluminescent Substrate"The rabbit antibody AAA46303 from produced very good results in western blotting with the lysate of human CD44-positive cancer cells as well as with the recombinant N-terminal protein. The detection was specific, resulting only in the protein bands of expected mol. weights. The antibody is also capable of detecting shed CD44 in conditioned medium. High specificity and stability. I would highly recommend this antibody for detection of CD44 in human cells by western blotting.")
IHC (Immunohiostchemistry)
(Anti- CD44 Picoband antibody, AAA46303, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti- CD44 Picoband antibody, AAA46303, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
WB (Western Blot)
(Anti- CD44 Picoband antibody, AAA46303, Western blottingAll lanes: Anti CD44 (AAA46303) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: PANC Whole Cell Lysate at 40ugPredicted bind size: 82KDObserved bind size: 115KD)
WB (Western Blot)
(Anti- CD44 Picoband antibody, AAA46303, Western blottingAll lanes: Anti CD44 (AAA46303) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: PANC Whole Cell Lysate at 40ugPredicted bind size: 82KDObserved bind size: 115KD)
Related Product Information for anti-CD44 antibody
Description: Rabbit IgG polyclonal antibody for CD44 antigen(CD44) detection. Tested with WB, IHC-P in Human.
Background: CD44 is also known as LHR or MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
Background: CD44 is also known as LHR or MC56. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.
References
1. Fagan-Solis KD, et al. Challenging the roles of CD44 and lipolysis stimulated lipoprotein receptor in conveying Clostridium perfringens iota toxin cytotoxicity in breast cancer. Mol Cancer, 2014 Jul 2. 2. Zhang P, et al. CD44 variant, but not standard CD44 isoforms, mediate disassembly of endothelial VE-cadherin junction on metastatic melanoma cells. FEBS Lett, 2014 Dec 20.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,635 Da
NCBI Official Full Name
CD44 antigen isoform 1
NCBI Official Synonym Full Names
CD44 molecule (Indian blood group)
NCBI Official Symbol
CD44
NCBI Official Synonym Symbols
IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
NCBI Protein Information
CD44 antigen
UniProt Protein Name
CD44 antigen
UniProt Gene Name
CD44
UniProt Synonym Gene Names
LHR; MDU2; MDU3; MIC4; ECMR-III; PGP-1; PGP-I
UniProt Entry Name
CD44_HUMAN
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Product Notes
The CD44 cd44 (Catalog #AAA46303) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD44 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD44 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CD44 cd44 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD44, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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