FCM/FACS (Flow Cytometry)
(CD69 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CD69 Polyclonal Antibody | anti-CD69 antibody
CD69 Antibody (Center)
Gene Names
CD69; AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CD69, Antibody; CD69 Antibody (Center); Early activation antigen CD69; Activation inducer molecule; AIM; BL-AC/P26; C-type lectin domain family 2 member C; EA1; Early T-cell activation antigen p60; GP32/28; Leukocyte surface antigen Leu-23; MLR-3; CD69; CLEC2C; anti-CD69 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CD69 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the Central region of human CD69.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
119-148
Sequence Length
199
Applicable Applications for anti-CD69 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CD69 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CD69 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(CD69 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CD69 antibody detected the CD69 protein (arrow).)
WB (Western Blot)
(CD69 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CD69 antibody detected the CD69 protein (arrow).)
Related Product Information for anti-CD69 antibody
This gene encodes a member of the calcium dependent lectin
superfamily of type II transmembrane receptors. Expression of the
encoded protein is induced upon activation of T lymphocytes, and
may play a role in proliferation. Furthermore, the protein may act
to transmit signals in natural killer cells and platelets.
Alternative splicing results in multiple transcript variants.
superfamily of type II transmembrane receptors. Expression of the
encoded protein is induced upon activation of T lymphocytes, and
may play a role in proliferation. Furthermore, the protein may act
to transmit signals in natural killer cells and platelets.
Alternative splicing results in multiple transcript variants.
Product Categories/Family for anti-CD69 antibody
References
Davila, S., et al. Genes Immun. (2010) In press :
Kolenko, P., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65 (PT 12), 1258-1260 (2009) :
Hu, M., et al. J. Mol. Recognit. 22(6):516-520(2009)
Radstake, T.R., et al. PLoS ONE 4 (6), E5981 (2009) :
Natarajan, K., et al. Biochemistry 39(48):14779-14786(2000)
Vance, B.A., et al. Arch. Biochem. Biophys. 368(2):214-220(1999)
Lopez-Cabrera, M., et al. J. Biol. Chem. 270(37):21545-21551(1995)
Bezouska, K., et al. Biochem. Biophys. Res. Commun. 208(1):68-74(1995)
Santis, A.G., et al. Eur. J. Immunol. 24(7):1692-1697(1994)
Lopez-Cabrera, M., et al. J. Exp. Med. 178(2):537-547(1993)
Ziegler, S.F., et al. Eur. J. Immunol. 23(7):1643-1648(1993)
Cambiaggi, C., et al. Immunogenetics 36(2):117-120(1992)
Kolenko, P., et al. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65 (PT 12), 1258-1260 (2009) :
Hu, M., et al. J. Mol. Recognit. 22(6):516-520(2009)
Radstake, T.R., et al. PLoS ONE 4 (6), E5981 (2009) :
Natarajan, K., et al. Biochemistry 39(48):14779-14786(2000)
Vance, B.A., et al. Arch. Biochem. Biophys. 368(2):214-220(1999)
Lopez-Cabrera, M., et al. J. Biol. Chem. 270(37):21545-21551(1995)
Bezouska, K., et al. Biochem. Biophys. Res. Commun. 208(1):68-74(1995)
Santis, A.G., et al. Eur. J. Immunol. 24(7):1692-1697(1994)
Lopez-Cabrera, M., et al. J. Exp. Med. 178(2):537-547(1993)
Ziegler, S.F., et al. Eur. J. Immunol. 23(7):1643-1648(1993)
Cambiaggi, C., et al. Immunogenetics 36(2):117-120(1992)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22559
NCBI Official Full Name
early activation antigen CD69
NCBI Official Synonym Full Names
CD69 molecule
NCBI Official Symbol
CD69
NCBI Official Synonym Symbols
AIM; EA1; MLR-3; CLEC2C; GP32/28; BL-AC/P26
NCBI Protein Information
early activation antigen CD69
UniProt Protein Name
Early activation antigen CD69
UniProt Gene Name
CD69
UniProt Synonym Gene Names
CLEC2C; AIM
UniProt Entry Name
CD69_HUMAN
Similar Products
Product Notes
The CD69 cd69 (Catalog #AAA288035) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 119-148. The CD69 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD69 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD69 cd69 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD69, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.