IHC (Immunohistochemisry)
(AAA323373 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit CD81 Polyclonal Antibody | anti-CD81 antibody
CD81 Antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CD81, Antibody; CD81 Antibody; 26 kDa cell surface protein TAPA 1; 26 kDa cell surface protein TAPA-1; 26 kDa cell surface protein TAPA1; CD 81; CD81; CD81 antigen (target of antiproliferative antibody 1); CD81 antigen; CD81 molecule; CD81_HUMAN; CVID6; S5.7; TAPA 1; TAPA1; Target of the antiproliferative antibody 1; Tetraspanin 28; Tetraspanin-28; Tetraspanin28; Tspan 28; Tspan-28; Tspan28; anti-CD81 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
CD81 antibody detects endogenous levels of total CD81
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
165
Applicable Applications for anti-CD81 antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human CD81, corresponding to a region within the internal amino acids.
Conjugation
Unconjugated
IHC (Immunohistochemisry)
(AAA323373 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA323373 at 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA323373 staining U-2 OS cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
IF (Immunofluorescence)
(AAA323373 staining U-2 OS cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using CD81 Antibody.Lane 1: Hela treated with blocking peptideLane 2: HelaLane 3: HepG2.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using CD81 Antibody.Lane 1: Hela treated with blocking peptideLane 2: HelaLane 3: HepG2.)
Related Product Information for anti-CD81 antibody
Function: May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV.
Subunit Structure: Homodimer (PubMed:20375010). Interacts directly with IGSF8 (PubMed:11504738). Interacts with CD53 and SCIMP (PubMed:21930792). Interacts with IFITM1 (PubMed:2398277). Interacts with IFITM2 and IFITM3 (By similarity). Part of a GPCR-tetraspanin complex consisting at least of ADGRG1, CD81, eventually CD9, and GNA11 in which CD81 enhances the association of ADGRG1 with GNA11 (PubMed:15004227). Interacts with CLDN1 (PubMed:20375010, PubMed:21516087). Interacts with CLDN6 and CLDN9 (PubMed:20375010). Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3 (PubMed:27993971).
Post-translational Modifications: Not glycosylated.
Similarity: Belongs to the tetraspanin (TM4SF) family.
Subunit Structure: Homodimer (PubMed:20375010). Interacts directly with IGSF8 (PubMed:11504738). Interacts with CD53 and SCIMP (PubMed:21930792). Interacts with IFITM1 (PubMed:2398277). Interacts with IFITM2 and IFITM3 (By similarity). Part of a GPCR-tetraspanin complex consisting at least of ADGRG1, CD81, eventually CD9, and GNA11 in which CD81 enhances the association of ADGRG1 with GNA11 (PubMed:15004227). Interacts with CLDN1 (PubMed:20375010, PubMed:21516087). Interacts with CLDN6 and CLDN9 (PubMed:20375010). Interacts (via the second extracellular domain) with integrin ITGAV:ITGB3 (PubMed:27993971).
Post-translational Modifications: Not glycosylated.
Similarity: Belongs to the tetraspanin (TM4SF) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26 kDa
NCBI Official Full Name
CD81 antigen isoform 2
NCBI Official Synonym Full Names
CD81 molecule
NCBI Official Symbol
CD81
NCBI Official Synonym Symbols
S5.7; CVID6; TAPA1; TSPAN28
NCBI Protein Information
CD81 antigen
UniProt Protein Name
CD81 antigen
UniProt Gene Name
CD81
UniProt Synonym Gene Names
TAPA1; TSPAN28; Tspan-28
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Product Notes
The CD81 cd81 (Catalog #AAA323373) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD81 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD81 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CD81 cd81 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD81, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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