Mouse CD81/TAPA-1 Monoclonal Antibody | anti-CD81 antibody

CD81/TAPA-1

Cat. Num. AAA214619

• Gene Names: CD81; S5.7; CVID6; TAPA1; TSPAN28
• Reactivity: Human, Mouse, Rat. Others not known.
• Applications: Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, Functional Assay
• Synonyms: CD81/TAPA-1, Antibody; CD81/TAPA-1; CD81; Target of the antiproliferative antibody 1 (TAPA-1); Tetraspanin-28; Tspan-28; anti-CD81 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: 1.3.3.22
• Specificity: Recognizes a protein of 26kDa, identified as CD81 (Workshop VI; Code CD81.1)). CD81 has a very broad cellular distribution, being expressed on T- and B-lymphocytes, NK cells, thymocytes, eosinophils, fibroblasts, epithelial and endothelial cells. Neutrophils, erythrocytes and platelets are negative, while monocytes are variably positive. CD81 is a member of a family of tetraspans transmembrane proteins, including CD9, CD37, CD53, CD63, and CD82. It associates with CD19, CD21, Leu 13, and integrins on cell membrane and is involved in signal transduction in B lymphocyte development and cell adhesion. CD81 also acts as a receptor for the envelope protein E2 of chronic hepatitis C virus. Antibodies to CD81 have anti-proliferative effects on different lymphoid cell lines, particularly those derived from large cell lymphomas.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 165

• Applicable Applications for anti-CD81 antibody: IHC (Immunohistochemistry), WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), FA (Functional Assay)
• Immunogen: Recombinant full-length human CD81 protein
• Cellular Localization: Cell Surface
• Positive Control: MCF-7, Ramos, Daudi or Jurkat cells. Human lymphocytes. Human tonsil or liver.
• Hu-chromosome Location: 11p15.5
• Positive Control: Ramos, Daudi, Raji, U266, HUT-78, HUT-102, Jurkat, HL-60, KG1a, A293, A431, MG63, and human lymphocytes. Tonsil or Liver.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Non-hazardous. No MSDS required.

Application Data

Application Data

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).)

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Lymph Node stained with CD81 Mouse Monoclonal Antibody (1.3.3.22).)

References

Kishimoto T et al. eds. Leukocyte Typing VI, p187-189, Garland Publishing, New York, 1997. Takahashi S et al. TAPA-1, the target of an antiproliferative antibody, is associated on the cell surface with the Leu-13 antigen. J Immunol 1990, 145(7):2207-2213. Mannion BA et al. Transmembrane-4 superfamily proteins CD81 (TAPA-1), CD82, CD63, and CD53 specifically associated with integrin eta´ï¢eta+/- (CD49d/CD29). J Immunol 1996, 157(5):2039-2047. Sato S et al. Regulation of B lymphocyte development and activation by the CD19/CD21/CD81/Leu 13 complex requires the cytoplasmic domain of CD19. J Immunol 1997, 159(7):3278-3287. Horvath G et al. CD19 is linked to the integrin-associated tetraspans CD9, CD81, and CD82. J Biol Chem 1998, 273(46):30537-30543. Todd SC et al. CD81 expressed on human thymocytes mediates integrin activation and interleukin 2-dependent proliferation. J Exp Med 1996, 184(5):2055-2060. Levy S et al. CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system. Annu Rev Immunol 1998, 16:89-109. Pileri P et al. Binding of hepatitis C virus to CD81. Science 1998, 282:938-941. Schlossman SF et al. eds. Leukocyte Typing V, Oxford University Press, Oxford, 1995.

NCBI and Uniprot Product Information

• NCBI GI #: 663071018
• NCBI GeneID: 975
• NCBI Accession #: NP_001284578.1
• NCBI GenBank Nucleotide #: NM_001297649.1
• UniProt Accession #: P60033; P18582; Q5U0J6
• Molecular Weight: 26kDa
• NCBI Official Full Name: CD81 antigen isoform 2
• NCBI Official Synonym Full Names: CD81 molecule
• NCBI Official Symbol: CD81
• NCBI Official Synonym Symbols: S5.7; CVID6; TAPA1; TSPAN28
• NCBI Protein Information: CD81 antigen
• UniProt Protein Name: CD81 antigen
• UniProt Gene Name: CD81
• UniProt Synonym Gene Names: TAPA1; TSPAN28; Tspan-28