FCM/FACS (Flow Cytometry)
(Cdc14 Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human, mouse Cdc14 Polyclonal Antibody | anti-CDC14A antibody
Cdc14 Antibody
Gene Names
CDC14A; cdc14; hCDC14
Reactivity
Human, mouse
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Cdc14, Antibody; Cdc14 Antibody; Dual specificity protein phosphatase CDC14A; CDC14 cell division cycle 14 homolog A; CDC14A; anti-CDC14A antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Cdc14 antibody is generated from rabbits immunized with a recombinant protein encoding aa 1~379 of human cdc14.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Length
610
Applicable Applications for anti-CDC14A antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Cdc14 Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Cdc14 Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Cdc14 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Cdc14 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Cdc14 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Cdc14 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(His6-cdc14 Antibody western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the cdc14 antibody detected the cdc14 protein (arrow).)
WB (Western Blot)
(His6-cdc14 Antibody western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the cdc14 antibody detected the cdc14 protein (arrow).)
Related Product Information for anti-CDC14A antibody
The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms.
Product Categories/Family for anti-CDC14A antibody
References
Kaiser,B.K., et al. Mol. Biol. Cell 13 (7), 2289-2300 (2002)
Mailand,N., et al. Nat. Cell Biol. 4 (4), 317-322 (2002)
Bembenek,J. and Yu,H. J. Biol. Chem. 276 (51), 48237-48242 (2001)
Li,L., et al. J. Biol. Chem. 275 (4), 2410-2414 (2000)
Wong,A.K., et al. Genomics 59 (2), 248-251 (1999)
Li,L., et al. J. Biol. Chem. 272 (47), 29403-29406 (1997)
Mailand,N., et al. Nat. Cell Biol. 4 (4), 317-322 (2002)
Bembenek,J. and Yu,H. J. Biol. Chem. 276 (51), 48237-48242 (2001)
Li,L., et al. J. Biol. Chem. 275 (4), 2410-2414 (2000)
Wong,A.K., et al. Genomics 59 (2), 248-251 (1999)
Li,L., et al. J. Biol. Chem. 272 (47), 29403-29406 (1997)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66574
NCBI Official Full Name
dual specificity protein phosphatase CDC14A isoform 1
NCBI Official Synonym Full Names
cell division cycle 14A
NCBI Official Symbol
CDC14A
NCBI Official Synonym Symbols
cdc14; hCDC14
NCBI Protein Information
dual specificity protein phosphatase CDC14A
UniProt Protein Name
Dual specificity protein phosphatase CDC14A
UniProt Gene Name
CDC14A
UniProt Entry Name
CC14A_HUMAN
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Product Notes
The CDC14A cdc14a (Catalog #AAA288431) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cdc14 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Cdc14 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CDC14A cdc14a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdc14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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