IHC (Immunohiostchemistry)
(AAA322736 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse CDC14B/C Polyclonal Antibody | anti-CDC14B antibody
CDC14B/C Antibody
Gene Names
CDC14B; CDC14B3; Cdc14B1; Cdc14B2; hCDC14B
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CDC14B/C, Antibody; CDC14B/C Antibody; CC14B_HUMAN; Cdc 14B; Cdc 14B1; Cdc 14B2; CDC 14B3; CDC14 cell division cycle 14 homolog B; CDC14 homolog B; Cdc14b; Cdc14B1; Cdc14B2; CDC14B3; Cell division cycle 14 homolog B; Dual specificity protein phosphatase CDC14B; hCDC 14B; hCDC14B; OTTHUMP00000063774; anti-CDC14B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CDC14B/C antibody detects endogenous levels of total CDC14B/C
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
461
Applicable Applications for anti-CDC14B antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CDC14B/C
Subcellular Location
Nucleus > Nucleolus. Nucleus > Nucleoplasm. Following DNA damage, translocates from the nucleolus to the nucleoplasm and interacts with FZR1/CDH1.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA322736 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA322736 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of CDC14B/C using HepG2 whole cell lysates)
WB (Western Blot)
(Western blot analysis of CDC14B/C using HepG2 whole cell lysates)
Related Product Information for anti-CDC14B antibody
Function: Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.
Subunit Structure: Interacts with FZR1/CDH1.
Similarity: Composed of two structurally equivalent A and B domains that adopt a dual specificity protein phosphatase (DSP) fold. Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.
Subunit Structure: Interacts with FZR1/CDH1.
Similarity: Composed of two structurally equivalent A and B domains that adopt a dual specificity protein phosphatase (DSP) fold. Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 60 kDa
Predicted: 57 kDa
Predicted: 57 kDa
NCBI Official Full Name
dual specificity protein phosphatase CDC14B isoform 3
NCBI Official Synonym Full Names
cell division cycle 14B
NCBI Official Symbol
CDC14B
NCBI Official Synonym Symbols
CDC14B3; Cdc14B1; Cdc14B2; hCDC14B
NCBI Protein Information
dual specificity protein phosphatase CDC14B
UniProt Protein Name
Dual specificity protein phosphatase CDC14B
UniProt Gene Name
CDC14B
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Product Notes
The CDC14B cdc14b (Catalog #AAA322736) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC14B/C Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CDC14B/C can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CDC14B cdc14b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC14B/C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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