WB (Western Blot)
(Detection of human CDC20 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, SR, K-562, and OVCAR-3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody (AAA211346 lot 3) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse CDC20 Polyclonal Antibody | anti-CDC20 antibody
Rabbit anti-CDC20 Antibody, Affinity Purified
Gene Names
CDC20; CDC20A; p55CDC; bA276H19.3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CDC20, Antibody; Rabbit anti-CDC20 Antibody, Affinity Purified; CDC20A; p55CDC; cell division cycle 20 homolog; bA276H19.3; cell division cycle protein 20 homolog; CDC20 cell division cycle 20 homolog; Cell division cycle protein 20 homolog; cell division cycle 20; anti-CDC20 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-CDC20 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Pig
Immunogen
Between 449 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CDC20 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, SR, K-562, and OVCAR-3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody (AAA211346 lot 3) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human CDC20 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, SR, K-562, and OVCAR-3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody (AAA211346 lot 3) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse CDC20 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody (AAA211346 lot 3) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse CDC20 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody (AAA211346 lot 3) used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody (AAA211346 lot 3) used for IP at 6 ug per reaction. CDC20 was also immunoprecipitated by a previous lot of this antibody (AAA211346 lot 2) and a second antibody against a different epitope of CDC20 . For blotting immunoprecipitated CDC20, AAA211346 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CDC20 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDC20 antibody (AAA211346 lot 3) used for IP at 6 ug per reaction. CDC20 was also immunoprecipitated by a previous lot of this antibody (AAA211346 lot 2) and a second antibody against a different epitope of CDC20 . For blotting immunoprecipitated CDC20, AAA211346 was used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-CDC20 antibody
CDC20 is a WD repeat containing cell cycle-regulator that binds to and activates the ubiquitin-ligase activity of the APC/c (anaphase-promoting complex/cyclosome). APC/c ubiquitinating activity is responsible for the degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. CDC20 has also been reported to form a complex with histone deacetylases and can function as a transcriptional repressor involved in cell cycle arrest. As a regulator of APC/c activity, CDC20 plays a key role in the spindle-assembly checkpoint.
Product Categories/Family for anti-CDC20 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,723 Da
NCBI Official Full Name
cell division cycle protein 20 homolog
NCBI Official Synonym Full Names
cell division cycle 20
NCBI Official Symbol
CDC20
NCBI Official Synonym Symbols
CDC20A; p55CDC; bA276H19.3
NCBI Protein Information
cell division cycle protein 20 homolog; cell division cycle 20 homolog; CDC20 cell division cycle 20 homolog
UniProt Protein Name
Cell division cycle protein 20 homolog
UniProt Gene Name
CDC20
UniProt Entry Name
CDC20_HUMAN
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Product Notes
The CDC20 cdc20 (Catalog #AAA211346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CDC20 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CDC20 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CDC20 cdc20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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