WB (Western Blot)
(Western blot analysis of CDC20 in lysates of Jurkat , using CDC20 Antibody(AF4759).)
Rabbit CDC20 Polyclonal Antibody | anti-CDC20 antibody
CDC20 Antibody
Gene Names
CDC20; CDC20A; p55CDC; bA276H19.3
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%)
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Synonyms
CDC20, Antibody; CDC20 Antibody; bA276H19.3; Cdc 20; CDC20; CDC20 cell division cycle 20 homolog; CDC20_HUMAN; CDC20A; Cell division cycle 20; Cell division cycle 20 homolog (S. cerevisiae); Cell division cycle 20 homolog; Cell division cycle protein 20 homolog; fizzy; MGC102824; p55CDC; anti-CDC20 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
CDC20 Antibody detects endogenous levels of total CDC20.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CDC20 antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CDC20, corresponding to a region within N-terminal amino acids.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Acetylated. Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C).Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2.Dephosphorylated by CTDP1.Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex.
Subunit Structure
Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with NEUROD2 and SPATC1 (By similarity). Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2 and SIRT2. Interacts with isoform 1 of NEK2. Interacts with HSF1 (via phosphorylated form); this interaction occurs in mitosis in a MAD2L1-dependent manner and prevents PLK1-stimulated degradation of HSF1 by blocking the recruitment of the SCF (BTRC) ubiquitin ligase complex. Interacts (via the N-terminal substrate-binding domain) with FBXO5 (By similarity).
Similarity
Belongs to the WD repeat CDC20/Fizzy family.
Subcellular Location
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of CDC20 in lysates of Jurkat , using CDC20 Antibody(AF4759).)
WB (Western Blot)
(Western blot analysis of CDC20 in lysates of Jurkat , using CDC20 Antibody(AF4759).)
WB (Western Blot)
(Western blot analysis of extracts from A2780 cells, using CDC20 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from A2780 cells, using CDC20 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-CDC20 antibody
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,723 Da
NCBI Official Full Name
cell division cycle protein 20 homolog
NCBI Official Synonym Full Names
cell division cycle 20
NCBI Official Symbol
CDC20
NCBI Official Synonym Symbols
CDC20A; p55CDC; bA276H19.3
NCBI Protein Information
cell division cycle protein 20 homolog; cell division cycle 20 homolog; CDC20 cell division cycle 20 homolog
UniProt Protein Name
Cell division cycle protein 20 homolog
UniProt Gene Name
CDC20
UniProt Entry Name
CDC20_HUMAN
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Product Notes
The CDC20 cdc20 (Catalog #AAA329559) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDC20 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's CDC20 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the CDC20 cdc20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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