IHC (Immunohiostchemistry)
(Anti-Cdc25B antibody, AAA45155, IHC(P)IHC(P): Human Lung Cancer Tissue)
Rabbit anti-Human, Rat Cdc25B Polyclonal Antibody | anti-CDC25B antibody
Anti-Cdc25B antibody
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Cdc25B, Antibody; Anti-Cdc25B antibody; M-phase inducer phosphatase 2; cell division cycle 25 homolog B(S. pombe); AI604853 antibody; Cdc 25B antibody; Cdc25b antibody; CDC25B1 antibody; CDC25B2 antibody; CDC25B3 antibody; CDC25HU2 antibody; Cdc25m2 antibody; Cell division cycle 25 homolog B antibody; Cell division cycle 25B antibody; Cell division cycle 25B isoform 1 antibody; Cell division cycle 25B isoform 2 antibody; Cell division cycle 25B isoform 3 antibody; Cell division cycle 25B isoform 4 antibody; Dual specificity phosphatase Cdc25B antibody; EC 3.1.3.48 antibody; M phase inducer phosphatase 2 antibody; M-phase inducer phosphatase 2 antibody; MPIP2_HUMAN antibody; anti-CDC25B antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
580
Applicable Applications for anti-CDC25B antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cdc25B (541-559aa DYRPMNHEAFKDELKTFRL), different from the related mouse sequence by three amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti-Cdc25B antibody, AAA45155, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-Cdc25B antibody, AAA45155, IHC(P)IHC(P): Human Lung Cancer Tissue)
WB (Western Blot)
(Anti-Cdc25B antibody, AAA45155, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: Rat Lung Tissue Lysate)
WB (Western Blot)
(Anti-Cdc25B antibody, AAA45155, Western blottingLane 1: Rat Brain Tissue LysateLane 2: Rat Kidney Tissue LysateLane 3: Rat Lung Tissue Lysate)
Related Product Information for anti-CDC25B antibody
Background: Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human CDC25 genes code for the A, B, and C forms of CDC25. CDC25B is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and CDC25C at serine-216; phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
M-phase inducer phosphatase 2
NCBI Official Synonym Full Names
cell division cycle 25B
NCBI Official Symbol
CDC25B
NCBI Protein Information
M-phase inducer phosphatase 2; dual specificity phosphatase Cdc25B
UniProt Protein Name
M-phase inducer phosphatase 2
UniProt Gene Name
CDC25B
UniProt Synonym Gene Names
CDC25HU2
UniProt Entry Name
MPIP2_HUMAN
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Product Notes
The CDC25B cdc25b (Catalog #AAA45155) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Cdc25B antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Cdc25B can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CDC25B cdc25b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cdc25B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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