FCM/FACS (Flow Cytometry)
(CDC5L Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CDC5L Polyclonal Antibody | anti-CDC5L antibody
CDC5L Antibody (Center)
Gene Names
CDC5L; CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CDC5L, Antibody; CDC5L Antibody (Center); Cell division cycle 5-like protein; Cdc5-like protein; Pombe cdc5-related protein; CDC5L; KIAA0432; PCDC5RP; anti-CDC5L antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CDC5L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-213 amino acids from the Central region of human CDC5L.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
187-213
Sequence Length
802
Applicable Applications for anti-CDC5L antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CDC5L Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CDC5L Antibody (Center) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of CDC5L Antibody (Center) in MCF-7 cell line lysates (35ug/lane). CDC5L (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CDC5L Antibody (Center) in MCF-7 cell line lysates (35ug/lane). CDC5L (arrow) was detected using the purified Pab.)
Related Product Information for anti-CDC5L antibody
The protein is a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.
References
Grillari,J., et.al., J. Biol. Chem. 284 (42), 29193-29204 (2009) Zhang,N., et.al., EMBO Rep. 10 (9), 1029-1035 (2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
92251
NCBI Official Full Name
cell division cycle 5-like protein
NCBI Official Synonym Full Names
cell division cycle 5-like
NCBI Official Symbol
CDC5L
NCBI Official Synonym Symbols
CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1
NCBI Protein Information
cell division cycle 5-like protein
UniProt Protein Name
Cell division cycle 5-like protein
UniProt Gene Name
CDC5L
UniProt Synonym Gene Names
KIAA0432; PCDC5RP; Cdc5-like protein
UniProt Entry Name
CDC5L_HUMAN
Similar Products
Product Notes
The CDC5L cdc5l (Catalog #AAA287530) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 187-213. The CDC5L Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDC5L can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CDC5L cdc5l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC5L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.