WB (Western Blot)
(Western blot analysis of Phospho-CDC5L(Tyr232) in lysates of HeLa, using Phospho-CDC5L(Tyr232) Antibody.)
Rabbit CDC5L Polyclonal Antibody | anti-CDC5L antibody
Phospho-CDC5L (Tyr232) Antibody
Gene Names
CDC5L; CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
ELISA, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
CDC5L, Antibody; Phospho-CDC5L (Tyr232) Antibody; CDC5; CDC5 cell division cycle 5-like (S. pombe); Cdc5 related protein; CDC5-LIKE; Cdc5-like protein; Cdc5l; CDC5L_HUMAN; CEF1; Cell division cycle 5 S. pombe homolog of; Cell division cycle 5-like protein; dJ319D22.1; KIAA0432; PCDC5RP; Pombe Cdc5 related protein; Pombe Cdc5-related protein; anti-CDC5L antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-CDC5L(Tyr232) Antibody detects endogenous levels of CDC5L only when phosphorylated at Tyr232.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
802
Applicable Applications for anti-CDC5L antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CDC5L around the phosphorylation site of Tyr232.
Tissue Specificity
Ubiquitously expressed in both fetal and adult tissues.
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-CDC5L(Tyr232) in lysates of HeLa, using Phospho-CDC5L(Tyr232) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-CDC5L(Tyr232) in lysates of HeLa, using Phospho-CDC5L(Tyr232) Antibody.)
IHC (Immunohiostchemistry)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining human kidney cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining human kidney cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-CDC5L antibody
Description: DNA-binding protein involved in cell cycle control. May act as a transcription activator. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR).
Post Translational Modifications: Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
Subunit Structure: Homodimer. Interacts with DAPK3 (By similarity). Belongs to the spliceosome complex. Part of a spliceosomal 'core' complex consisting of CDC5L, PLRG1, SPF27, CCAP1, CCAP3 and CCAP6. Interacts with PLRG1, Lodestar/TTF2, and NIPP1/PPP1R8. Identified in the spliceosome C complex. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its C-terminus) directly in the complex with PRPF19 and BCAS2. Interacts (via its C-terminus) directly with PRGL1 (via its WD40 repeat domain); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts with CTNNBL1. Interacts with PRPF19 (via N-terminus) (By similarity).
Similarity: Belongs to the CEF1 family.
Post Translational Modifications: Phosphorylated on serine and threonine residues. Phosphorylation on Thr-411 and Thr-438 is required for CDC5L-mediated mRNA splicing. Has no effect on subcellular location nor on homodimerization. Phosphorylated in vitro by CDK2. Phosphorylation enhances interaction with PPP1R8.
Subunit Structure: Homodimer. Interacts with DAPK3 (By similarity). Belongs to the spliceosome complex. Part of a spliceosomal 'core' complex consisting of CDC5L, PLRG1, SPF27, CCAP1, CCAP3 and CCAP6. Interacts with PLRG1, Lodestar/TTF2, and NIPP1/PPP1R8. Identified in the spliceosome C complex. Component of the PRP19-CDC5L splicing complex composed of a core complex comprising a homotetramer of PRPF19, CDC5L, PLRG1 and BCAS2, and at least three less stably associated proteins CTNNBL1, CWC15 and HSPA8. Interacts (via its C-terminus) directly in the complex with PRPF19 and BCAS2. Interacts (via its C-terminus) directly with PRGL1 (via its WD40 repeat domain); the interaction is required for mRNA splicing but not for spliceosome assembly. Also interacts with CTNNBL1. Interacts with PRPF19 (via N-terminus) (By similarity).
Similarity: Belongs to the CEF1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 92 kDa
Predicted: 93 kDa
Predicted: 93 kDa
NCBI Official Full Name
Cell division cycle 5-like protein
NCBI Official Synonym Full Names
cell division cycle 5 like
NCBI Official Symbol
CDC5L
NCBI Official Synonym Symbols
CDC5; CEF1; PCDC5RP; CDC5-LIKE; dJ319D22.1
NCBI Protein Information
cell division cycle 5-like protein
UniProt Protein Name
Cell division cycle 5-like protein
UniProt Gene Name
CDC5L
UniProt Synonym Gene Names
KIAA0432; PCDC5RP; Cdc5-like protein
UniProt Entry Name
CDC5L_HUMAN
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Product Notes
The CDC5L cdc5l (Catalog #AAA328260) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CDC5L (Tyr232) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's CDC5L can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the CDC5L cdc5l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC5L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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