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product-image-AAA324220_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from 293, using CDH3 Ab.)

Rabbit anti-Human CDH3 Polyclonal Antibody | anti-CDH3 antibody

CDH3 Antibody

Gene Names
CDH3; CDHP; HJMD; PCAD
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin. (Thermo Fisher Scientific).
Synonyms
CDH3, Antibody; CDH3 Antibody; CADH3_HUMAN; Cadherin 3; Cadherin 3 precursor; Cadherin 3 type 1; Cadherin-3; Cadp; Calcium dependent adhesion protein placental; CDH 3; CDH3; CDH3 protein; CDHP; HJMD; P cadherin (placental); P-cadherin; PCAD; Placental cadherin; anti-CDH3 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin. (Thermo Fisher Scientific).
Form/Format
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
~1mg/ml (varies by lot)
Sequence Length
784
Applicable Applications for anti-CDH3 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CDH3, corresponding to a region within N-terminal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.

WB (Western Blot)

(Western blot analysis of extracts from 293, using CDH3 Ab.)

product-image-AAA324220_WB13.jpg WB (Western Blot) (Western blot analysis of extracts from 293, using CDH3 Ab.)

IF (Immunofluorescence)

(AAA324220 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C.Samples were then incubated with primary Ab(AAA324220) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab.The nuclear counter stain is DAPI (blue).)

product-image-AAA324220_IF15.jpg IF (Immunofluorescence) (AAA324220 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C.Samples were then incubated with primary Ab(AAA324220) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab.The nuclear counter stain is DAPI (blue).)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
86 KD
NCBI Official Full Name
cadherin-3 isoform 2
NCBI Official Synonym Full Names
cadherin 3
NCBI Official Symbol
CDH3
NCBI Official Synonym Symbols
CDHP; HJMD; PCAD
NCBI Protein Information
cadherin-3
UniProt Protein Name
Cadherin-3
UniProt Gene Name
CDH3
UniProt Synonym Gene Names
CDHP; P-cadherin

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Product Notes

The CDH3 cdh3 (Catalog #AAA324220) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDH3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDH3 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CDH3 cdh3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDH3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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