WB (Western Blot)
(All lanes : Anti-CDK5R1 Antibody (N-term) at 1:2000 dilutionLane 1: human cerebellum lysatesLane 2: mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, mouse CDK5R1 Polyclonal Antibody | anti-CDK5R1 antibody
CDK5R1 Antibody (N-term)
Gene Names
CDK5R1; p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
Reactivity
Human, mouse
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CDK5R1, Antibody; CDK5R1 Antibody (N-term); Cyclin-dependent kinase 5 activator 1; CDK5 activator 1; Cyclin-dependent kinase 5 regulatory subunit 1; TPKII regulatory subunit; p35; p25; Tau protein kinase II 23 kDa subunit; p23; CDK5R1; CDK5R; NCK5A; anti-CDK5R1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CDK5R1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 30-64 amino acids from the N-terminal region of human CDK5R1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.309 (varies by lot)
Sequence Length
307
Applicable Applications for anti-CDK5R1 antibody
ELISA, WB (Western Blot)
Cellular Location
Cyclin-dependent kinase 5 activator 1, p35: Cell membrane; Lipid-anchor; Cytoplasmic side. Note=In the primary cortical neurons, p35 is present in the peripheries and nerve terminals
Tissue Location
Brain and neuron specific.
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-CDK5R1 Antibody (N-term) at 1:2000 dilutionLane 1: human cerebellum lysatesLane 2: mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-CDK5R1 Antibody (N-term) at 1:2000 dilutionLane 1: human cerebellum lysatesLane 2: mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-CDK5R1 antibody
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. Activator of TPKII. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution.
Product Categories/Family for anti-CDK5R1 antibody
References
Tsai L.-H.,et al.Nature 371:419-423(1994).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Patrick G.N.,et al.Nature 402:615-622(1999).
Kerokoski P.,et al.Brain Res. Mol. Brain Res. 106:50-56(2002).
Kalnine N.,et al.Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Patrick G.N.,et al.Nature 402:615-622(1999).
Kerokoski P.,et al.Brain Res. Mol. Brain Res. 106:50-56(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34060
NCBI Official Full Name
cyclin-dependent kinase 5 activator 1
NCBI Official Synonym Full Names
cyclin-dependent kinase 5, regulatory subunit 1 (p35)
NCBI Official Symbol
CDK5R1
NCBI Official Synonym Symbols
p23; p25; p35; CDK5R; NCK5A; CDK5P35; p35nck5a
NCBI Protein Information
cyclin-dependent kinase 5 activator 1
UniProt Protein Name
Cyclin-dependent kinase 5 activator 1
UniProt Gene Name
CDK5R1
UniProt Synonym Gene Names
CDK5R; NCK5A; CDK5 activator 1; p35; p25; p23
UniProt Entry Name
CD5R1_HUMAN
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Product Notes
The CDK5R1 cdk5r1 (Catalog #AAA289454) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDK5R1 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CDK5R1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CDK5R1 cdk5r1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDK5R1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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