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IHC (Immunohistochemistry)
(Figure 4 Immunohistochemistry Validation of CDKN2A in Human Colon TissueImmunohistochemical analysis of paraffin-embedded human colon tissue using anti-CDKN2A antibody (8975) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
Rabbit anti-Human CDKN2A Polyclonal Antibody | anti-CDKN2A antibody
CDKN2A Antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
CDKN2A Antibody is affinity chromatography purified via peptide column
Synonyms
CDKN2A, Antibody; CDKN2A Antibody; cyclin-dependent kinase inhibitor 2A (melanoma; p16; inhibits CDK4); CDKN2A Antibody: ARF; MLM; P14; P16; P19;cmM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P19ARF; P16INK4; P16INK4A; P16-INK4A; Cyclin-dependent kinase 4 inhibitor A; anti-CDKN2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
CDKN2A Antibody is affinity chromatography purified via peptide column
Form/Format
Liquid
CDKN2A Antibody is supplied in PBS containing 0.02% sodium azide.
CDKN2A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-CDKN2A antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Source
Human
Immunogen
Anti-CDKN2A antibody (8975) was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human CDKN2A.
The immunogen is located within the last 50 amino acids of CDKN2A.
The immunogen is located within the last 50 amino acids of CDKN2A.
Isoforms
Human CDKN2A has 6 isoforms, including isoform 1 (156aa, 17kD), isoform 2 (105aa, 11kD), isoform 3 (116aa, 12kD), isoform 5 (167aa, 18kD), isoform tumor suppressor ARF (132aa, 14kD) and isoform smARF (85aa, 9kD).
Mouse CDKN2A has 4 isoforms, including isoform 1 (168aa, 18kD), isoform 2 (125aa, 14kD), isoform tumor suppressor ARF (169aa, 19kD) and isoform smARF (125aa, 14kD).
Rat CDKN2A has two isoforms, including isoform 1 (159aa, 17kD) and isoform ARF (160aa, 17kD).
8975 can detect only human isoforms 1, 2 and 5, but cannot detect mouse and rat.
Mouse CDKN2A has 4 isoforms, including isoform 1 (168aa, 18kD), isoform 2 (125aa, 14kD), isoform tumor suppressor ARF (169aa, 19kD) and isoform smARF (125aa, 14kD).
Rat CDKN2A has two isoforms, including isoform 1 (159aa, 17kD) and isoform ARF (160aa, 17kD).
8975 can detect only human isoforms 1, 2 and 5, but cannot detect mouse and rat.
Conjugation
Unconjugated
Validation Note
KO Validation (Figure 1): Anti-CDKN2A antibodies (8975) specificity was further verified by CDKN2A specific knockout. CDKN2A signal was not detected in CDKN2A knockout 293 cells as compared to that in control wild type cells. Independent Antibody Validation in Cell lines (Figure 2) shows similar CDKN2A expression profile in human cell lines detected by two independent anti-CDKN2A antibodies that recognize different epitopes, 4211 against N-terminus domain and 8975 against the C-terminus domain. CDKN2A proteins are detected only in 293, HeLa and HepG2 cell lines by the two independent antibodies
Preparation and Storage
CDKN2A antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
IHC (Immunohistochemistry)
(Figure 4 Immunohistochemistry Validation of CDKN2A in Human Colon TissueImmunohistochemical analysis of paraffin-embedded human colon tissue using anti-CDKN2A antibody (8975) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IHC (Immunohistochemistry)
(Figure 4 Immunohistochemistry Validation of CDKN2A in Human Colon TissueImmunohistochemical analysis of paraffin-embedded human colon tissue using anti-CDKN2A antibody (8975) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
WB (Western Blot)
(Figure 3 Western Blot Validation in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: CDKN2A, 8975 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 3 Western Blot Validation in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: CDKN2A, 8975 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Application Data
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: CDKN2A, 4211 (2 μg/mL), CDKN2A, 8975 (2 μg/mL) and beta-actin, 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
Application Data
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: CDKN2A, 4211 (2 μg/mL), CDKN2A, 8975 (2 μg/mL) and beta-actin, 3779 (1 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 1 KO Validation of CDKN2A in 293 CellsLoading: 10 μg of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, 8975 (2 μg/mL) and beta-actin, 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 1 KO Validation of CDKN2A in 293 CellsLoading: 10 μg of 293 WT cell lysates or CDKN2A KO cell lysates. Antibodies: CDKN2A, 8975 (2 μg/mL) and beta-actin, 3779 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
Related Product Information for anti-CDKN2A antibody
CDKN2A Antibody: The CDKN2A locus gives rise to 2 distinct transcripts from different promoters. The transcripts have been designated p16(INK4A) and p14(ARF). This chromosomal region undergoes a number of inversions, translocations, heterozygous deletions, and homozygous deletions in a variety of malignant cell lines including those from glioma, non-small cell lung cancer, leukemia, and melanoma. Deletion of the region containing CDKN2A is found in more than half of all melanoma cell lines. Conversely, transfection of CDKN2A suppressed the growth of two independent mesothelioma cell lines, suggesting that inactivation of the CDKN2 gene is an essential step in the etiology of malignant mesotheliomas. CDKN2A induces a G1 cell cycle arrest by inhibiting the phosphorylation of the Rb protein by the cyclin-dependent kinases CDK4 and CDK6. CDKN2A is expressed as at least three distinct isoforms.
Product Categories/Family for anti-CDKN2A antibody
References
Stone et al. Cancer Res. 1995; 55:2988-94.
Kamb et al. Nature Genet. 1994; 8:22-6.
Kratzke et al. J. Nat. Cancer Inst. 1995; 87:1870-5.
Stott et al. EMBO J. 1998; 17:5001-14.
Kamb et al. Nature Genet. 1994; 8:22-6.
Kratzke et al. J. Nat. Cancer Inst. 1995; 87:1870-5.
Stott et al. EMBO J. 1998; 17:5001-14.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 17 kDa
Observed: 20 kDa
Observed: 20 kDa
NCBI Official Full Name
cyclin-dependent kinase inhibitor 2A isoform p16INK4a
NCBI Official Synonym Full Names
cyclin-dependent kinase inhibitor 2A
NCBI Official Symbol
CDKN2A
NCBI Official Synonym Symbols
ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
NCBI Protein Information
cyclin-dependent kinase inhibitor 2A; CDKN2A; CDK4 inhibitor p16-INK4; multiple tumor suppressor 1; cell cycle negative regulator beta; cyclin-dependent kinase 4 inhibitor A; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4)
UniProt Protein Name
Cyclin-dependent kinase inhibitor 2A, isoforms 1/2/3
UniProt Gene Name
CDKN2A
UniProt Synonym Gene Names
CDKN2; MTS1; CDK4I; MTS-1; p16-INK4; p16INK4A
UniProt Entry Name
CD2A1_HUMAN
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Product Notes
The CDKN2A cdkn2a (Catalog #AAA40919) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CDKN2A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CDKN2A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the CDKN2A cdkn2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDKN2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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