FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-CDT1/DUP antibody (AAA126102).Overlay histogram showing HepG2 cells stained with AAA126102 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDT1/DUP Antibody (AAA126102, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit CDT1/DUP Polyclonal Antibody | anti-CDT1 antibody
Anti-CDT1/DUP Antibody Picoband
Gene Names
CDT1; DUP; RIS2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay, ELISA
Purity
Immunogen affinity purified.
Synonyms
CDT1/DUP, Antibody; Anti-CDT1/DUP Antibody Picoband; anti-CDT1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Natural and recombinant Human IL6
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-CDT1 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA
Samples
Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Detection Range
4.69 pg/ml-300 pg/ml
Immunogen
E Coli-derived human CDT1/DUP recombinant protein (Position: D508-L546).
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-CDT1/DUP antibody (AAA126102).Overlay histogram showing HepG2 cells stained with AAA126102 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDT1/DUP Antibody (AAA126102, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of HepG2 cells using anti-CDT1/DUP antibody (AAA126102).Overlay histogram showing HepG2 cells stained with AAA126102 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CDT1/DUP Antibody (AAA126102, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of CDT1/DUP using anti-CDT1/DUP antibody (AAA126102).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human Raji whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat liver tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDT1/DUP antigen affinity purified polyclonal antibody (#AAA126102) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDT1/DUP at approximately 65 kDa. The expected band size for CDT1/DUP is at 60 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of CDT1/DUP using anti-CDT1/DUP antibody (AAA126102).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human Raji whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat liver tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDT1/DUP antigen affinity purified polyclonal antibody (#AAA126102) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDT1/DUP at approximately 65 kDa. The expected band size for CDT1/DUP is at 60 kDa.)
Related Product Information for anti-CDT1 antibody
Background: The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.
Product Categories/Family for anti-CDT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
546
NCBI Official Full Name
DNA replication factor Cdt1
NCBI Official Synonym Full Names
chromatin licensing and DNA replication factor 1
NCBI Official Symbol
CDT1
NCBI Official Synonym Symbols
DUP; RIS2
NCBI Protein Information
DNA replication factor Cdt1; CDT1; Double parked, Drosophila, homolog of
UniProt Protein Name
DNA replication factor Cdt1
UniProt Gene Name
CDT1
UniProt Synonym Gene Names
DUP
UniProt Entry Name
CDT1_HUMAN
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Product Notes
The CDT1 cdt1 (Catalog #AAA126102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CDT1/DUP Antibody Picoband reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDT1/DUP can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the CDT1 cdt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDT1/DUP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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