Human DNA replication factor Cdt1 ELISA Kit | CDT1 elisa kit

Human DNA replication factor Cdt1 ELISA Kit

Cat. Num. AAA100479

• Gene Names: CDT1; DUP; RIS2
• Reactivity: Human
• Synonyms: DNA replication factor Cdt1; N/A; Human DNA replication factor Cdt1 ELISA Kit; CDT1/DNA replication factor Cdt1/Double parked homolog/DUP/RIS2; CDT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CDT1. No significant cross-reactivity or interference between CDT1 and analogues was observed.
• Sequence Length: 546
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CDT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CDT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for CDT1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CDT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- CDT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CDT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CDT1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 188497689
• NCBI GeneID: 81620
• NCBI Accession #: NP_112190.2
• NCBI GenBank Nucleotide #: NM_030928.3
• Molecular Weight: 60,390 Da
• NCBI Official Full Name: DNA replication factor Cdt1
• NCBI Official Synonym Full Names: chromatin licensing and DNA replication factor 1
• NCBI Official Symbol: CDT1
• NCBI Official Synonym Symbols: DUP; RIS2
• NCBI Protein Information: DNA replication factor Cdt1
• UniProt Protein Name: DNA replication factor Cdt1
• UniProt Gene Name: CDT1
• UniProt Synonym Gene Names: DUP
• UniProt Entry Name: CDT1_HUMAN