Rabbit anti-Human CEBPA Polyclonal Antibody | anti-CEBPA antibody

CEBPA Antibody

Cat. Num. AAA232437

• Gene Names: CEBPA; CEBP; C/EBP-alpha
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: >95%, Protein G purified
• Synonyms: CEBPA, Antibody; CEBPA Antibody; CCAAT/enhancer-binding protein alpha; CEBPA; anti-CEBPA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 239

• Applicable Applications for anti-CEBPA antibody: IHC (Immunohistochemistry), ELISA
• Immunogen: Recombinant Human CCAAT/enhancer-binding protein alpha protein (1-124AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-21820 / sc-365318 / sc-61 / sc-9314 / sc-9315
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

WB (Western Blot)

(Western BlotPositive WB detected in: A549 whole cell lysate,HepG2 whole cell lysate,MCF-7 whole cell lysateAll lanes: CEBPA antibody at 2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38,36,26,42 KDaObserved band size: 38 KDa)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human pancreatic tissue using AAA232437 at dilution of 1:100)

Related Product Information for anti-CEBPA antibody

Transcription factor that coordinates proliferation arrest and the differentiation of myeloid progenitors, adipocytes, hepatocytes, and cells of the lung and the placenta. Binds directly to the consensus DNA sequence 5'-T[TG]NNGNAA[TG]-3' acting as an activator on distinct target genes (PubMed: 11242107). During early embryogenesis, plays essential and redundant functions with CEBPB. Essential for the transition from common myeloid progenitors (CMP) to granulocyte/monocyte progenitors (GMP). Critical for the proper development of the liver and the lung (By similarity). Necessary for terminal adipocyte differentiation, is required for postnatal maintenance of systemic energy homeostasis and lipid storage (By similarity). To regulate these different processes at the proper moment and tissue, interplays with other transcription factors and modulators. Downregulates the expression of genes that maintain cells in an undifferentiated and proliferative state through E2F1 repression, which is critical for its ability to induce adipocyte and granulocyte terminal differentiation. Reciprocally E2F1 blocks adipocyte differentiation by binding to specific promoters and repressing CEBPA binding to its target gene promoters. Proliferation arrest also depends on a functional binding to SWI/SNF complex (PubMed: 14660596). In liver, regulates gluconeogenesis and lipogenesis through different mechanisms. To regulate gluconeogenesis, functionally cooperates with FOXO1 binding to IRE-controlled promoters and regulating the expression of target genes such as PCK1 or G6PC. To modulate lipogenesis, interacts and transcriptionally synergizes with SREBF1 in promoter activation of specific lipogenic target genes such as ACAS2. In adipose tissue, seems to act as FOXO1 coactivator accessing to ADIPOQ promoter through FOXO1 binding sites (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 551894998
• NCBI GeneID: 1050
• NCBI Accession #: NP_001272758.1
• NCBI GenBank Nucleotide #: NM_001285829.1
• UniProt Accession #: P49715; P78319; Q05CA4; A7LNP2
• Molecular Weight: 41,249 Da
• NCBI Official Full Name: CCAAT/enhancer-binding protein alpha isoform b
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein alpha
• NCBI Official Symbol: CEBPA
• NCBI Official Synonym Symbols: CEBP; C/EBP-alpha
• NCBI Protein Information: CCAAT/enhancer-binding protein alpha
• UniProt Protein Name: CCAAT/enhancer-binding protein alpha
• UniProt Gene Name: CEBPA
• UniProt Synonym Gene Names: C/EBP alpha
• UniProt Entry Name: CEBPA_HUMAN