WB (Western Blot)
(Western Blot; Sample: Lane1: U937 cell lysate; Lane2: THP1 cell lysatePrimary Ab: 0.2ug/ml Mouse Anti-Rat CEBPa AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Mouse anti-Human, Rat CCAAT/Enhancer Binding Protein Alpha (CEBPa) Monoclonal Antibody | anti-CEBPa antibody
Monoclonal Antibody to CCAAT/Enhancer Binding Protein Alpha (CEBPa)
Gene Names
Cebpa; DBPCEP
Reactivity
Human, Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Protein A + Protein G affinity chromatography
Synonyms
CCAAT/Enhancer Binding Protein Alpha (CEBPa), Antibody; Monoclonal Antibody to CCAAT/Enhancer Binding Protein Alpha (CEBPa); CCAAT/Enhancer Binding Protein Alpha; CEBP-A; anti-CEBPa antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Clone Number
C2
Specificity
The antibody is a mouse monoclonal antibody raised against CEBPa. It has been selected for its ability to recognize CEBPa in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid. 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence Positions
Ala72~Ala358
Applicable Applications for anti-CEBPa antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Recombinant CCAAT/Enhancer Binding Protein Alpha (CEBPa)
Preparation and Storage
Store at 4 degree C for frequent use. Store at -20 degree C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stable for 12 months if correctly stored.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Western Blot; Sample: Lane1: U937 cell lysate; Lane2: THP1 cell lysatePrimary Ab: 0.2ug/ml Mouse Anti-Rat CEBPa AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: U937 cell lysate; Lane2: THP1 cell lysatePrimary Ab: 0.2ug/ml Mouse Anti-Rat CEBPa AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: U937 cell lysate; Lane2: THP1 cell lysate Primary Ab: 0.2ug/ml Mouse Anti-Rat CEBPa Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: U937 cell lysate; Lane2: THP1 cell lysate Primary Ab: 0.2ug/ml Mouse Anti-Rat CEBPa Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Product Categories/Family for anti-CEBPa antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,371 Da
NCBI Official Full Name
CCAAT/enhancer-binding protein alpha isoform a
NCBI Official Synonym Full Names
CCAAT/enhancer binding protein (C/EBP), alpha
NCBI Official Symbol
Cebpa
NCBI Official Synonym Symbols
DBPCEP
NCBI Protein Information
CCAAT/enhancer-binding protein alpha; C/EBP alpha; CCAAT/enhancer binding protein, alpha; CAAT/enhancer-binding protein DNA-binding protein; CAAT/enhancer-binding protein, DNA-binding protein
UniProt Protein Name
CCAAT/enhancer-binding protein alpha
UniProt Gene Name
Cebpa
UniProt Synonym Gene Names
C/EBP alpha
UniProt Entry Name
CEBPA_RAT
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Product Notes
The CEBPa cebpa (Catalog #AAA152942) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ala72~Ala358. The Monoclonal Antibody to CCAAT/Enhancer Binding Protein Alpha (CEBPa) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CCAAT/Enhancer Binding Protein Alpha (CEBPa) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CEBPa cebpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CCAAT/Enhancer Binding Protein Alpha (CEBPa), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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