FCM/FACS (Flow Cytometry)
(CEBPA Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit CEBPA Polyclonal Antibody | anti-CEBPA antibody
CEBPA Antibody (C-term)
Gene Names
CEBPA; CEBP; C/EBP-alpha
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CEBPA, Antibody; CEBPA Antibody (C-term); CCAAT/enhancer-binding protein alpha; C/EBP alpha; CEBPA; anti-CEBPA antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CEBPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-330 amino acids from the C-terminal region of human CEBPA.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
304-330
Sequence Length
393
Applicable Applications for anti-CEBPA antibody
WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(CEBPA Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(CEBPA Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of CEBPA Antibody (C-term) in mouse liver tissue lysates (35ug/lane). CEBPA (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CEBPA Antibody (C-term) in mouse liver tissue lysates (35ug/lane). CEBPA (arrow) was detected using the purified Pab.)
Related Product Information for anti-CEBPA antibody
CEBPA is a bZIP transcription factor which can bind as a homodimer to certain promoters and enhancers. It can also form heterodimers with the related proteins CEBP-beta and CEBP-gamma. This protein has been shown to bind to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Also, the protein can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells.
Product Categories/Family for anti-CEBPA antibody
References
Twu, Y.C., et al. Blood 115(12):2491-2499(2010)
?Nanri, T., et al. Genes Chromosomes Cancer 49(3):237-241(2010)
?Burnett, A.K., et al. Blood 115(5):948-956(2010)
?Dufour, A., et al. J. Clin. Oncol. 28(4):570-577(2010)
?Nanri, T., et al. Genes Chromosomes Cancer 49(3):237-241(2010)
?Burnett, A.K., et al. Blood 115(5):948-956(2010)
?Dufour, A., et al. J. Clin. Oncol. 28(4):570-577(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37561
NCBI Official Full Name
CCAAT/enhancer-binding protein alpha isoform a
NCBI Official Synonym Full Names
CCAAT/enhancer binding protein (C/EBP), alpha
NCBI Official Symbol
CEBPA
NCBI Official Synonym Symbols
CEBP; C/EBP-alpha
NCBI Protein Information
CCAAT/enhancer-binding protein alpha
UniProt Protein Name
CCAAT/enhancer-binding protein alpha
UniProt Gene Name
CEBPA
UniProt Synonym Gene Names
C/EBP alpha
UniProt Entry Name
CEBPA_HUMAN
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Product Notes
The CEBPA cebpa (Catalog #AAA285315) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 304-330. The CEBPA Antibody (C-term) reacts with Human, mouse (Predicted Reactivity: Bovine, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's CEBPA can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CEBPA cebpa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEBPA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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