Rabbit CEBPB Polyclonal Antibody | anti-CEBPB antibody

CEBPB Antibody (C-term)

Cat. Num. AAA285541

• Gene Names: CEBPB; TCF5; IL6DBP; NF-IL6; C/EBP-beta
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CEBPB, Antibody; CEBPB Antibody (C-term); CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein; LAP; Liver-enriched inhibitory protein; LIP; Nuclear factor NF-IL6; Transcription factor 5; TCF-5; CEBPB; TCF5; anti-CEBPB antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CEBPB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-298 amino acids from the C-terminal region of human CEBPB.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 271-298

• Applicable Applications for anti-CEBPB antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Function: Important transcription factor regulating theexpression of genes involved in immune andinflammatory responses (PubMed: , PubMed: ,PubMed: , PubMed:18647749). Playsalso a significant role in adipogenesis, as wellas in the gluconeogenic pathway, liverregeneration, and hematopoiesis. Theconsensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacityis governed by protein interactions andpost-translational protein modifications.During early embryogenesis, plays essentialand redundant functions with CEBPA. Has apromitotic effect on many cell types such ashepatocytes and adipocytes but has anantiproliferative effect on T-cells byrepressing MYC expression, facilitatingdifferentiation along the T-helper 2 lineage.Binds to regulatory regions of severalacute-phase and cytokines genes and plays arole in the regulation of acute-phase reactionand inflammation. Plays also a role inintracellular bacteria killing (By similarity).During adipogenesis, is rapidly expressedand, after activation by phosphorylation,induces CEBPA and PPARG, which turn on theseries of adipocyte genes that give rise to theadipocyte phenotype. The delayedtransactivation of the CEBPA and PPARGgenes by CEBPB appears necessary to allowmitotic clonal expansion and therebyprogression of terminal differentiation(PubMed: ). Essentialfor female reproduction because of a criticalrole in ovarian follicle development (Bysimilarity). Restricts osteoclastogenesis:together with NFE2L1; represses expressionof DSPP during odontoblast differentiation (Bysimilarity).
• Cellular Location: Nucleus. Cytoplasm. Note=Translocates tothe nucleus when phosphorylated at Ser-288.In T-cells when sumoylated drawn topericentric heterochromatin thereby allowingproliferation (By similarity).{ECO:0000250|UniProtKB:P28033,ECO:0000269|PubMed:9374525
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(CEBPB Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CEBPB Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of CEBPB (arrow) using rabbit polyclonal CEBPB Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CEBPB gene (Lane 2).)

Related Product Information for anti-CEBPB antibody

CEBPB is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related proteins CEBP-alpha, CEBP-delta, and CEBP-gamma. This protein is important in the regulation of genes involved in immune and inflammatory responses and has been shown to bind to the IL-1 response element in the IL-6 gene, as well as to regulatory regions of several acute-phase and cytokine genes. In addition, It can bind the promoter and upstream element and stimulate the expression of the collagen type I gene.

Product Categories/Family for anti-CEBPB antibody

Cardiovascular

References

Buck,M., et.al., Mol. Cell 4 (6), 1087-1092 (1999)

NCBI and Uniprot Product Information

• NCBI GI #: 28872796
• NCBI GeneID: 1051
• NCBI Accession #: NP_005185.2
• NCBI GenBank Nucleotide #: NM_005194.3
• UniProt Accession #: P17676; Q96IH2; Q9H4Z5; A8K671
• Molecular Weight: 36106
• NCBI Official Full Name: CCAAT/enhancer-binding protein beta isoform a
• NCBI Official Synonym Full Names: CCAAT/enhancer binding protein (C/EBP), beta
• NCBI Official Symbol: CEBPB
• NCBI Official Synonym Symbols: TCF5; IL6DBP; NF-IL6; C/EBP-beta
• NCBI Protein Information: CCAAT/enhancer-binding protein beta
• UniProt Protein Name: CCAAT/enhancer-binding protein beta
• UniProt Gene Name: CEBPB
• UniProt Synonym Gene Names: LAP; LIP; TCF-5
• UniProt Entry Name: CEBPB_HUMAN