IF (Immunofluorescence)
(AAA323913 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab(AAA323913) and mouse anti-betatubulin Ab for 1 hour at 37°C. An AlexaFluor594conjugated goat anti-rabbit IgG(H+L) Ab(Red) and anAlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
CEBPG Polyclonal Antibody | anti-CEBPG antibody
CEBPG Antibody
Gene Names
CEBPG; GPE1BP; IG/EBP-1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Synonyms
CEBPG, Antibody; CEBPG Antibody; C/EBP gamma; CCAAT/enhancer binding protein (C/EBP) gamma; CCAAT/enhancer binding protein gamma; CCAAT/enhancer-binding protein gamma; CEBPG; CEBPG_HUMAN; GPE1BP; IG/EBP 1; IG/EBP1; anti-CEBPG antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
CEBPG Antibody detects endogenous levels of total CEBPG.
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CEBPG antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human CEBPG, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C.
IF (Immunofluorescence)
(AAA323913 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab(AAA323913) and mouse anti-betatubulin Ab for 1 hour at 37°C. An AlexaFluor594conjugated goat anti-rabbit IgG(H+L) Ab(Red) and anAlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
IF (Immunofluorescence)
(AAA323913 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were thenincubated with primary Ab(AAA323913) and mouse anti-betatubulin Ab for 1 hour at 37°C. An AlexaFluor594conjugated goat anti-rabbit IgG(H+L) Ab(Red) and anAlexaFluor488 conjugated goat anti-mouse IgG(H+L)Ab(Green) were used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA323913 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA323913 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using CEBPG Antibody.Lane 1: A549 cells, blocked with antigen-specific peptides,Lane 2: A549 cells,Lane 3: Mouse spleen,Lane 4: Hela cells,Lane 5: P19 cells.)
WB (Western Blot)
(Western blot analysis of extracts from various samples, using CEBPG Antibody.Lane 1: A549 cells, blocked with antigen-specific peptides,Lane 2: A549 cells,Lane 3: Mouse spleen,Lane 4: Hela cells,Lane 5: P19 cells.)
Related Product Information for anti-CEBPG antibody
Transcription factor that binds to the promoter and the enhancer regions of target genes. Binds to the enhancer element PRE-I (positive regulatory element-I) of the IL-4 gene (PubMed:7665092). Binds to the promoter and the enhancer of the immunoglobulin heavy chain. Binds to GPE1, a cis-acting element in the G-CSF gene promoter.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22 KD
NCBI Official Full Name
CCAAT/enhancer-binding protein gamma
NCBI Official Synonym Full Names
CCAAT enhancer binding protein gamma
NCBI Official Symbol
CEBPG
NCBI Official Synonym Symbols
GPE1BP; IG/EBP-1
NCBI Protein Information
CCAAT/enhancer-binding protein gamma
UniProt Protein Name
CCAAT/enhancer-binding protein gamma
UniProt Gene Name
CEBPG
UniProt Synonym Gene Names
C/EBP gamma
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Product Notes
The CEBPG cebpg (Catalog #AAA323913) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CEBPG Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CEBPG can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CEBPG cebpg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEBPG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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