WB (Western Blot)
(Anti-CELA2A Antibody (C-term) at 1:1000 dilution + human pancreas lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit CELA2A Polyclonal Antibody | anti-CELA2A antibody
CELA2A Antibody (C-term)
Gene Names
CELA2A; PE-1; ELA2A
Reactivity
Human (Predicted Reactivity: Pig)
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CELA2A, Antibody; CELA2A Antibody (C-term); Chymotrypsin-like elastase family member 2A; Elastase-2A; CELA2A; ELA2A; anti-CELA2A antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Pig)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CELA2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 231-260 amino acids from the C-terminal region of human CELA2A.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
231-260
Sequence Length
269
Applicable Applications for anti-CELA2A antibody
ELISA, WB (Western Blot)
Function
Elastase that enhances insulin signaling and might have a physiologic role in cellular glucose metabolism. Circulates in plasma and reduces platelet hyperactivation, triggers both insulin secretion and degradation, and increases insulin sensitivity.
Cellular Location
Secreted.
Tissue Location
Expressed in pancreas. Not detected in keratinocytes (PubMed:10620133). Detected in exocrine secretions of the pancreas (at protein level). Also expressed in a small fraction of cells in pancreatic islets, adrenal cortex, intestinal glands and colonic lymphoid follicles (at protein level) (PubMed:31358993) Detected in plasma (PubMed:31358993).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Anti-CELA2A Antibody (C-term) at 1:1000 dilution + human pancreas lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CELA2A Antibody (C-term) at 1:1000 dilution + human pancreas lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-CELA2A antibody
Elastases form a subfamily of serine proteases that
hydrolyze many proteins in addition to elastin. Humans have six
elastase genes which encode the structurally similar proteins
elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human
elastases, elastase 2A is secreted from the pancreas as a zymogen.
In other species, elastase 2A has been shown to preferentially
cleave proteins after leucine, methionine, and phenylalanine
residues.
hydrolyze many proteins in addition to elastin. Humans have six
elastase genes which encode the structurally similar proteins
elastase 1, 2, 2A, 2B, 3A, and 3B. Like most of the human
elastases, elastase 2A is secreted from the pancreas as a zymogen.
In other species, elastase 2A has been shown to preferentially
cleave proteins after leucine, methionine, and phenylalanine
residues.
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Lausen, J., et al. Oncogene 25(9):1349-1357(2006)
Szepessy, E., et al. Pancreatology 6 (1-2), 117-122 (2006) :
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Lausen, J., et al. Oncogene 25(9):1349-1357(2006)
Szepessy, E., et al. Pancreatology 6 (1-2), 117-122 (2006) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
chymotrypsin-like elastase family member 2A preproprotein
NCBI Official Synonym Full Names
chymotrypsin-like elastase family, member 2A
NCBI Official Symbol
CELA2A
NCBI Official Synonym Symbols
PE-1; ELA2A
NCBI Protein Information
chymotrypsin-like elastase family member 2A
UniProt Protein Name
Chymotrypsin-like elastase family member 2A
UniProt Gene Name
CELA2A
UniProt Synonym Gene Names
ELA2A
UniProt Entry Name
CEL2A_HUMAN
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Product Notes
The CELA2A cela2a (Catalog #AAA289653) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 231-260. The CELA2A Antibody (C-term) reacts with Human (Predicted Reactivity: Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's CELA2A can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CELA2A cela2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CELA2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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