IHC (Immunohiostchemistry)
(AAA323385 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse CENPU Polyclonal Antibody | anti-CENPU antibody
CENPU Antibody
Gene Names
CENPU; KLIP1; PBIP1; CENP50; MLF1IP; CENPU50
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CENPU, Antibody; CENPU Antibody; CENP U; CENP-50; CENP-U; CENP50; CENPU; CENPU_HUMAN; CENPU50; centromere protein of 50 kDa; Centromere protein U; FLJ23468; HHV8 LNAIP1; ICEN24; Interphase centromere complex protein 24; Kaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1; KLIP-1; KLIP1; KSHV latent nuclear antigen interacting protein 1; KSHV latent nuclear antigen-interacting protein 1; KSHV LNAIP1; LNAIP 1; LNAIP-1; MLF1-interacting protein; MLF1IP; MLF1IP1; PBIP1; Polo-box-interacting protein 1; anti-CENPU antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CENPU antibody detects endogenous levels of total CENPU
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
418
Applicable Applications for anti-CENPU antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Nucleus. Chromosome > Centromere > Kinetochore. Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates.
Tissue Specificity
Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.
Conjugation
Unconjugated
IHC (Immunohiostchemistry)
(AAA323385 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA323385 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of CENPU expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of CENPU expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-CENPU antibody
Description: Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.
Subunit Structure: Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts with the N-terminal domain of Kaposi's sarcoma-associated herpesvirus latent nuclear antigen (LNA). Interacts with MLF1. Interacts with PLK1.
Post-translational Modifications: Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.
Similarity: Belongs to the CENP-U/AME1 family.
Subunit Structure: Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. Interacts with the N-terminal domain of Kaposi's sarcoma-associated herpesvirus latent nuclear antigen (LNA). Interacts with MLF1. Interacts with PLK1.
Post-translational Modifications: Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.
Similarity: Belongs to the CENP-U/AME1 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 48 kDa
Predicted: 48 kDa
Predicted: 48 kDa
NCBI Official Full Name
centromere protein U
NCBI Official Synonym Full Names
centromere protein U
NCBI Official Symbol
CENPU
NCBI Official Synonym Symbols
KLIP1; PBIP1; CENP50; MLF1IP; CENPU50
NCBI Protein Information
centromere protein U
UniProt Protein Name
Centromere protein U
UniProt Gene Name
CENPU
UniProt Synonym Gene Names
ICEN24; KLIP1; MLF1IP; PBIP1; CENP-U; CENP-50
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Product Notes
The CENPU cenpu (Catalog #AAA323385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CENPU Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CENPU can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CENPU cenpu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENPU, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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