WB (Western Blot)
(Anti-MLF1IP Antibody - Western Blot. Western blot of affinity purified anti-MLF1IP antibody shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (Lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for PBIP1/MLF1IP. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.)
Rabbit CENPU / MLF1IP Polyclonal Antibody | anti-CENPU antibody
Rabbit Polyclonal to Human CENPU / MLF1IP
Gene Names
CENPU; KLIP1; PBIP1; CENP50; MLF1IP; CENPU50
Reactivity
Bovine, Chimpanzee, Dog, Human
Applications
ELISA, Immunoprecipitation, Western Blot, Immunohistochemistry
Purity
Immunoaffinity Purified
Synonyms
CENPU / MLF1IP, Antibody; Rabbit Polyclonal to Human CENPU / MLF1IP; Anti-CENPU / MLF1IP Antibody (phospho-Thr78) IHC-plus; CENPU; CENP-U; CENP50; CENPU50; Centromere protein U; ICEN24; KLIP1; Polo-box-interacting protein 1; MLF1-interacting protein; CENP-50; Centromere protein of 50 kDa; MLF1 interacting protein; MLF1IP; PBIP1; Human CENPU; anti-CENPU antibody
Host
Rabbit
Reactivity
Bovine, Chimpanzee, Dog, Human
Clonality
Polyclonal
Specificity
Amino acids surrounding Thr78 of human MLF1IP protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
418
Applicable Applications for anti-CENPU antibody
ELISA, IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry)
Target Species
Human
Immunogen Description
Synthetic peptide from human MLF1IP / PBIP1.
Immunogen Type
Synthetic peptide
Immunogen
CENPU / MLF1IP antibody was raised against synthetic peptide from human MLF1IP / PBIP1.
Antigen Modification
pThr78
Preparation and Storage
Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.
WB (Western Blot)
(Anti-MLF1IP Antibody - Western Blot. Western blot of affinity purified anti-MLF1IP antibody shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (Lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for PBIP1/MLF1IP. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.)
WB (Western Blot)
(Anti-MLF1IP Antibody - Western Blot. Western blot of affinity purified anti-MLF1IP antibody shows detection of MLF1IP (arrowhead) in HeLa cells transfected with ZZ-tagged MLF1IP (Lane 1). Lane 2 is lysate from non-transfected HeLa cells, and Lane 3 is lysate from HeLa cells containing a knock-out mutation for PBIP1/MLF1IP. Personal Communication, Kyung S. Lee, CCR-NCI, Bethesda, MD.)
IHC (Immunohistochemistry)
(Anti-MLF1IP / PBIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
IHC (Immunohistochemistry)
(Anti-MLF1IP / PBIP1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Related Product Information for anti-CENPU antibody
Myeloid leukemia factor-1 (MLF1) Interacting protein, also known as PBIP1, is a novel gene that encodes a putative transcriptional repressor. It is localized to human chromosome 4q35.1, is expressed in both the nuclei and cytoplasm of cells, and is a novel substrate for polo-like kinase (PLK1). Phosphorylation by PLK1 regulates the PLK1-MLF1 interaction and localization to the kinetochores promoting proper chromosome segregation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,102 Da
NCBI Official Full Name
centromere protein U
NCBI Official Synonym Full Names
centromere protein U
NCBI Official Symbol
CENPU
NCBI Official Synonym Symbols
KLIP1; PBIP1; CENP50; MLF1IP; CENPU50
NCBI Protein Information
centromere protein U; MLF1 interacting protein; MLF1-interacting protein; centromere protein of 50 kDa; polo-box-interacting protein 1; interphase centromere complex protein 24; KSHV latent nuclear antigen interacting protein 1; KSHV latent nuclear antigen-interacting protein 1
UniProt Protein Name
Centromere protein U
UniProt Gene Name
CENPU
UniProt Synonym Gene Names
ICEN24; KLIP1; MLF1IP; PBIP1; CENP-U; CENP-50
UniProt Entry Name
CENPU_HUMAN
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Product Notes
The CENPU cenpu (Catalog #AAA50754) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human CENPU / MLF1IP reacts with Bovine, Chimpanzee, Dog, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CENPU / MLF1IP can be used in a range of immunoassay formats including, but not limited to, ELISA, IP (Immunoprecipitation), WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the CENPU cenpu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENPU / MLF1IP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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