IHC (Immunohiostchemistry)
((LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with CER1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using 0.025mg/ml of CER1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human CER1 Polyclonal Antibody | anti-CER1 antibody
CER1 Antibody (N-term)
Gene Names
CER1; DAND4
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CER1, Antibody; CER1 Antibody (N-term); Cerberus; Cerberus-related protein; DAN domain family member 4; CER1; DAND4; anti-CER1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human CER1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
56-85
Sequence Length
267
Applicable Applications for anti-CER1 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
((LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with CER1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using 0.025mg/ml of CER1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
((LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with CER1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Flow cytometric analysis of NCI-H292 cells using 0.025mg/ml of CER1 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of CER1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). CER1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CER1 Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). CER1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-CER1 antibody
CER1 is a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity.
Product Categories/Family for anti-CER1 antibody
References
Tang,P.L., et.al., J. Bone Miner. Res. 24 (6), 1013-1021 (2009)
Swinkels,M.E., et.al., Am. J. Med. Genet. A 146A (11), 1430-1438 (2008)
Swinkels,M.E., et.al., Am. J. Med. Genet. A 146A (11), 1430-1438 (2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30084
NCBI Official Full Name
cerberus
NCBI Official Synonym Full Names
cerberus 1, DAN family BMP antagonist
NCBI Official Symbol
CER1
NCBI Official Synonym Symbols
DAND4
NCBI Protein Information
cerberus
UniProt Protein Name
Cerberus
UniProt Gene Name
CER1
UniProt Synonym Gene Names
DAND4
UniProt Entry Name
CER1_HUMAN
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Product Notes
The CER1 cer1 (Catalog #AAA287436) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 56-85. The CER1 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CER1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CER1 cer1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CER1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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