Rabbit CH25H Polyclonal Antibody | anti-CH25H antibody

CH25H Antibody (N-Term)

Cat. Num. AAA290852

• Gene Names: CH25H; C25H
• Reactivity: Human, Mouse; Predicted: Pig
• Applications: ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: CH25H, Antibody; CH25H Antibody (N-Term); Cholesterol 25-hydroxylase; 1.14.99.38; Cholesterol 25-monooxygenase; h25OH; CH25H; anti-CH25H antibody

• Host: Rabbit
• Reactivity: Human, Mouse; Predicted: Pig
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CH25H antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 47-76 amino acids from human CH25H.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 272

• Applicable Applications for anti-CH25H antibody: ELISA, WB (Western Blot)
• Function: Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells (By similarity). May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (PubMed:9852097). As an interferon- stimulated gene, has broad antiviral activities against a wide range of enveloped viruses, such as vesicular stomatitis virus (VSV) and SARS coronavirus-2 (SARS-CoV-2). Its product, 25-hydroxycholesterol, activates the ER-localized enzyme ACAT to induce internalization of accessible cholesterol on the plasma membrane and restricts SARS-CoV-2 S protein-mediated fusion which inhibits virus replication (PubMed:33239446, PubMed:32944968). In testis, production of 25- hydroxycholesterol by macrophages plays a role in Leydig cell differentiation (By similarity).
• Cellular Location: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9Z0F5}; Multi-pass membrane protein {ECO:0000250|UniProtKB:Q9Z0F5}
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes : Anti-CH25H Antibody (N-Term) at 1:1000-1:2000 dilutionLane 1: Human brain lysateLane 2: Mouse heart lysateLane 3: Mouse lung lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 32 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-CH25H antibody

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.

Product Categories/Family for anti-CH25H antibody

Primary Antibodies; Cancer; Cardiovascular; Cell Biology

References

Lund E.G.,et al.J. Biol. Chem. 273:34316-34327 (1998). Ota T.,et al.Nat. Genet. 36:40-45 (2004). Deloukas P.,et al.Nature 429:375-381 (2004). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Riemenschneider M.,et al.Neurobiol. Aging 25:1305-1308 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4502499
• NCBI GeneID: 9023
• NCBI Accession #: NP_003947.1
• NCBI GenBank Nucleotide #: NM_003956.3
• UniProt Accession #: O95992; B2RBY3
• Molecular Weight: Calculated MW: 31745 Da
• NCBI Official Full Name: cholesterol 25-hydroxylase
• NCBI Official Synonym Full Names: cholesterol 25-hydroxylase
• NCBI Official Symbol: CH25H
• NCBI Official Synonym Symbols: C25H
• NCBI Protein Information: cholesterol 25-hydroxylase
• UniProt Protein Name: Cholesterol 25-hydroxylase
• UniProt Gene Name: CH25H
• UniProt Synonym Gene Names: h25OH