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WB (Western Blot)
(WB Suggested Anti-CHEK1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Small Intestine)
Rabbit CHEK1 Polyclonal Antibody | anti-CHEK1 antibody
CHEK1 antibody - middle region
Gene Names
CHEK1; CHK1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
CHEK1; Polyclonal Antibody; CHEK1 antibody - middle region; anti-CHEK1 antibody
Host
Rabbit
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Sequence
Synthetic peptide located within the following region: SARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDF
Sequence Length
476
Applicable Applications for anti-CHEK1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Homology
Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human CHEK1
Preparation and Storage
For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(WB Suggested Anti-CHEK1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Small Intestine)
WB (Western Blot)
(WB Suggested Anti-CHEK1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Small Intestine)
WB (Western Blot)
(Host: RabbitTarget Name: CHEK1Sample Type: Human Fetal MuscleAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: CHEK1Sample Type: Human Fetal MuscleAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: CHEK1Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: CHEK1Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: CHEK1Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: CHEK1Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)
WB (Western Blot)
(Host: RabbitTarget Name: CHEK1Sample Type: 721_BAntibody Dilution: 1.0ug/mlCHEK1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)
WB (Western Blot)
(Host: RabbitTarget Name: CHEK1Sample Type: 721_BAntibody Dilution: 1.0ug/mlCHEK1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)
WB (Western Blot)
(Sample Type: SH-SY5Y, HEK293TLanes :Lane 1: 40ug SH-SY5Y lysateLane 2: 40ug SH-SY5Y lysateLane 3: 40ug HEK293T lysatePrimary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit-HRPSecondary Antibody Dilution :1:8000Gene Name :CHEK1Submitted by :Anonymous)
WB (Western Blot)
(Sample Type: SH-SY5Y, HEK293TLanes :Lane 1: 40ug SH-SY5Y lysateLane 2: 40ug SH-SY5Y lysateLane 3: 40ug HEK293T lysatePrimary Antibody Dilution :1:1000Secondary Antibody :Anti-rabbit-HRPSecondary Antibody Dilution :1:8000Gene Name :CHEK1Submitted by :Anonymous)
IHC (Immunohistochemistry)
(Rabbit Anti-CHEK1 antibodyParaffin Embedded Tissue: Human Heart cellCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)
IHC (Immunohistochemistry)
(Rabbit Anti-CHEK1 antibodyParaffin Embedded Tissue: Human Heart cellCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)
Related Product Information for anti-CHEK1 antibody
This is a rabbit polyclonal antibody against CHEK1. It was validated on Western Blot using a cell lysate as a positive control.
Target Description: CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.
Target Description: CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.
Product Categories/Family for anti-CHEK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54kDa
NCBI Official Full Name
serine/threonine-protein kinase Chk1 isoform 1
NCBI Official Synonym Full Names
checkpoint kinase 1
NCBI Official Symbol
CHEK1
NCBI Official Synonym Symbols
CHK1
NCBI Protein Information
serine/threonine-protein kinase Chk1
UniProt Protein Name
Serine/threonine-protein kinase Chk1
UniProt Gene Name
CHEK1
UniProt Synonym Gene Names
CHK1
UniProt Entry Name
CHK1_HUMAN
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Product Notes
The CHEK1 chek1 (Catalog #AAA23414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHEK1 antibody - middle region reacts with Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CHEK1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the CHEK1 chek1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: SARITIPDIK KDRWYNKPLK KGAKRPRVTS GGVSESPSGF SKHIQSNLDF. It is sometimes possible for the material contained within the vial of "CHEK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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