Rabbit anti-Human CHEK1 Polyclonal Antibody | anti-CHEK1 antibody

CHEK1(Phospho-Ser317) antibody

Cat. Num. AAA305390

• Gene Names: CHEK1; CHK1
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: CHEK1, Antibody; CHEK1(Phospho-Ser317) antibody; CHK1; anti-CHEK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of CHEK1 only when phosphorylated at serine 317.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 476

• Applicable Applications for anti-CHEK1 antibody: IF (Immunofluorescence), WB (Western Blot)
• Immunogen Type: Peptide
• Immunogen Description: A phospho specific peptide corresponding to residues surrounding S317 of human CHEK1.
• Modification: Phospho-Ser317
• Target Name: CHEK1
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cell using Phospho-CHEK1-S317 antibody. Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.)

IF (Immunofluorescence)

(Immunofluorescence analysis of MCF7 cell using Phospho-CHEK1-S317 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts of MCF7 cell line, using Phospho-CHEK1-S317 antibody.)

Related Product Information for anti-CHEK1 antibody

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.

Product Categories/Family for anti-CHEK1 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 166295194
• NCBI GeneID: 1111
• NCBI Accession #: NP_001107593.1
• NCBI GenBank Nucleotide #: NM_001114121.2
• UniProt Accession #: O14757; A8K934; B4DDD0; B4DSK3; B5BTY6; F5H7S4; H2BI51
• Molecular Weight: 50,415 Da
• NCBI Official Full Name: serine/threonine-protein kinase Chk1 isoform 1
• NCBI Official Synonym Full Names: checkpoint kinase 1
• NCBI Official Symbol: CHEK1
• NCBI Official Synonym Symbols: CHK1
• NCBI Protein Information: serine/threonine-protein kinase Chk1
• UniProt Protein Name: Serine/threonine-protein kinase Chk1
• UniProt Gene Name: CHEK1
• UniProt Synonym Gene Names: CHK1
• UniProt Entry Name: CHK1_HUMAN