IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using Phospho-CHEK1-S280 antibody. Blue: DAPI for nuclear staining.)
Rabbit anti-Human, Mouse CHEK1-S280 Antibody | anti-CHEK1 antibody
Phospho-CHEK1-S280 Polyclonal Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
CHEK1-S280; N/A; Phospho-CHEK1-S280 Polyclonal Antibody; Phospho-CHEK1-S280: CHK1; anti-CHEK1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Concentration
1mg/ml (varies by lot)
Sequence Length
476
Applicable Applications for anti-CHEK1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Species
Human
Route
Synthetic Peptide
Immunogen
A phospho specific peptide corresponding to residues surrounding S280 of human CHEK1
Calculated Molecular Weight
54kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using Phospho-CHEK1-S280 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of MCF-7 cells using Phospho-CHEK1-S280 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of HT-29 cells, using Phospho-CHEK1-Ser280 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
WB (Western Blot)
(Western blot analysis of extracts of HT-29 cells, using Phospho-CHEK1-Ser280 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
Related Product Information for anti-CHEK1 antibody
The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
Product Categories/Family for anti-CHEK1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
476
NCBI Official Full Name
Serine/threonine-protein kinase Chk1
NCBI Official Synonym Full Names
checkpoint kinase 1
NCBI Official Symbol
CHEK1
NCBI Official Synonym Symbols
CHK1
NCBI Protein Information
serine/threonine-protein kinase Chk1; Chk1-S; checkpoint kinase-1; CHK1 checkpoint homolog; cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1
UniProt Protein Name
Serine/threonine-protein kinase Chk1
UniProt Gene Name
CHEK1
UniProt Synonym Gene Names
CHK1
UniProt Entry Name
CHK1_HUMAN
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Product Notes
The CHEK1 chek1 (Catalog #AAA36658) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CHEK1-S280 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CHEK1-S280 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CHEK1 chek1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHEK1-S280, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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