IP (Immunoprecipitation)
(Detection of human CHK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHK2 antibodies BL2880 (Cat. No. BL2881 (Cat. No. AAA211055) and BL2882 (Cat. No. used at 3 ug/mg lysate. For blotting immunoprecipitated CHK2, BL2882 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time 30 seconds.)
Rabbit anti-Human Chk2 Polyclonal Antibody | anti-Chk2 antibody
Rabbit anti-Chk2 Antibody, Affinity Purified
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
Chk2, Antibody; Rabbit anti-Chk2 Antibody, Affinity Purified; hCds1; serine/threonine-protein kinase Chk2; RAD53; PP1425; LFS2; HuCds1; CHK2; checkpoint-like protein CHK2; Checkpoint kinase 2; CHK2 checkpoint homolog; CDS1; cds1 homolog; Serine/threonine-protein kinase Chk2; checkpoint kinase 2; anti-Chk2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Chk2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 493 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human CHK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHK2 antibodies BL2880 (Cat. No. BL2881 (Cat. No. AAA211055) and BL2882 (Cat. No. used at 3 ug/mg lysate. For blotting immunoprecipitated CHK2, BL2882 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CHK2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHK2 antibodies BL2880 (Cat. No. BL2881 (Cat. No. AAA211055) and BL2882 (Cat. No. used at 3 ug/mg lysate. For blotting immunoprecipitated CHK2, BL2882 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human CHK2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CHK2 (Cat. No. AAA211055 Lot1)) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human CHK2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CHK2 (Cat. No. AAA211055 Lot1)) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-Chk2 antibody
Chk2 functions as a checkpoint protein kinase in response to DNA damage to maintain genomic stability. The yeast homologs are RAD53 and Cds1. The two major upstream controllers of Chk2 are ATM and ATR which are part of the phosphatidylinositol 3-kinase family. When activated, Chk2 targets p53, Brca1, Cdc25A and Cdc25C to cause cell cycle arrest. Chk2 is also known as serine/threonine-protein kinase CHK2, CHEK2, CDS1, LFS2, RAD53, HuCds1, and PP1425.
Product Categories/Family for anti-Chk2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,157 Da
NCBI Official Full Name
serine/threonine-protein kinase Chk2 isoform a
NCBI Official Synonym Full Names
checkpoint kinase 2
NCBI Official Symbol
CHEK2
NCBI Official Synonym Symbols
CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
NCBI Protein Information
serine/threonine-protein kinase Chk2; cds1 homolog; CHK2 checkpoint homolog; checkpoint-like protein CHK2
UniProt Protein Name
Serine/threonine-protein kinase Chk2
UniProt Gene Name
CHEK2
UniProt Synonym Gene Names
CDS1; CHK2; RAD53; Hucds1; hCds1
UniProt Entry Name
CHK2_HUMAN
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Product Notes
The Chk2 chek2 (Catalog #AAA211055) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Chk2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Chk2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the Chk2 chek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chk2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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