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IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug CHEK2 antibody. Western blot was performed from the immunoprecipitate using CHEK2 antibody at a dilition of 1:1000.)
Rabbit anti-Human, Mouse Chk2 Polyclonal Antibody | anti-CHEK2 antibody
Chk2 Rabbit pab Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Affinity purification
Synonyms
Chk2, Antibody; Chk2 Rabbit pab Antibody; CHEK2; Chk2 ; CHEK2 ; CDS1; CHK2 ; HuCds1 ; LFS2 ; PP1425; RAD53 ; Serine/threonine-protein kinase; Cds1 ;; anti-CHEK2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
586
Applicable Applications for anti-CHEK2 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A recombinant protein fragment of human Chk2
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol,
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug CHEK2 antibody. Western blot was performed from the immunoprecipitate using CHEK2 antibody at a dilition of 1:1000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of MCF-7 cells using 1ug CHEK2 antibody. Western blot was performed from the immunoprecipitate using CHEK2 antibody at a dilition of 1:1000.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using CHEK2 antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using CHEK2 antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using CHEK2 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using CHEK2 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using CHEK2 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded human lung cancer using CHEK2 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using CHEK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using CHEK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-CHEK2 antibody
Rabbit polyclonal to Human Chk2
Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain (8).
Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain (8).
References
1. Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415. 2. Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665. 3. Murakami, H. and Okayama, H. (1995) Nature 374, 817-819. 4. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186. 5. Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394. 6. Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765. 7. Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936. 8. Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
543
NCBI Official Full Name
serine/threonine-protein kinase Chk2 isoform c
NCBI Official Synonym Full Names
checkpoint kinase 2
NCBI Official Symbol
CHEK2
NCBI Official Synonym Symbols
CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
NCBI Protein Information
serine/threonine-protein kinase Chk2; cds1 homolog; CHK2 checkpoint homolog; checkpoint-like protein CHK2
UniProt Protein Name
Serine/threonine-protein kinase Chk2
UniProt Gene Name
CHEK2
UniProt Synonym Gene Names
CDS1; CHK2; RAD53; Hucds1; hCds1
UniProt Entry Name
CHK2_HUMAN
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Product Notes
The CHEK2 chek2 (Catalog #AAA36668) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Chk2 Rabbit pab Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Chk2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CHEK2 chek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chk2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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