IF (Immunofluorescence)
(Immunofluorescent analysis of CHMP2B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Rabbit anti-Human, Mouse CHMP2B Polyclonal Antibody | anti-CHMP2B antibody
CHMP2B Antibody
Gene Names
CHMP2B; DMT1; ALS17; VPS2B; VPS2-2; CHMP2.5
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
CHMP2B, Antibody; CHMP2B Antibody; DMT1; ALS17; VPS2B; VPS2-2; CHMP2.5; anti-CHMP2B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total CHMP2B protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
172
Applicable Applications for anti-CHMP2B antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human CHMP2B.
Target Name
CHMP2B
Preparation and Storage
Store at -20 degree C
IF (Immunofluorescence)
(Immunofluorescent analysis of CHMP2B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
IF (Immunofluorescence)
(Immunofluorescent analysis of CHMP2B staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CHMP2B staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of CHMP2B staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Western blot analysis of CHMP2B expression in MCF7 (A),LOVO (B), A549 (C), mouse lung (D), mouse kidney (E) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of CHMP2B expression in MCF7 (A),LOVO (B), A549 (C), mouse lung (D), mouse kidney (E) whole cell lysates.)
Related Product Information for anti-CHMP2B antibody
This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration.
Product Categories/Family for anti-CHMP2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24Kd
NCBI Official Full Name
charged multivesicular body protein 2b isoform 2
NCBI Official Synonym Full Names
charged multivesicular body protein 2B
NCBI Official Symbol
CHMP2B
NCBI Official Synonym Symbols
DMT1; ALS17; VPS2B; VPS2-2; CHMP2.5
NCBI Protein Information
charged multivesicular body protein 2b
UniProt Protein Name
Charged multivesicular body protein 2b
UniProt Gene Name
CHMP2B
UniProt Synonym Gene Names
CHMP2b; Vps2-2; hVps2-2
UniProt Entry Name
CHM2B_HUMAN
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Product Notes
The CHMP2B chmp2b (Catalog #AAA304370) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CHMP2B Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CHMP2B can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CHMP2B chmp2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHMP2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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