IHC (Immunohiostchemistry)
(CHRNA3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHRNA3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit CHRNA3 Polyclonal Antibody | anti-CHRNA3 antibody
CHRNA3 Antibody (N-term)
Gene Names
CHRNA3; LNCR2; PAOD2; NACHRA3
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CHRNA3, Antibody; CHRNA3 Antibody (N-term); Neuronal acetylcholine receptor subunit alpha-3; CHRNA3; NACHRA3; anti-CHRNA3 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CHRNA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-52 amino acids from the N-terminal region of human CHRNA3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
25-52
Sequence Length
489
Applicable Applications for anti-CHRNA3 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(CHRNA3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHRNA3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(CHRNA3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHRNA3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of CHRNA3 Antibody (N-term) in CEM cell line lysates (35ug/lane).CHRNA3 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of CHRNA3 Antibody (N-term) in CEM cell line lysates (35ug/lane).CHRNA3 (arrow) was detected using the purified Pab.)
Related Product Information for anti-CHRNA3 antibody
CHRNA3 locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.
Product Categories/Family for anti-CHRNA3 antibody
References
Cho, M.H., et al. Nat. Genet. 42(3):200-202(2010)
Girard, N., et al. Clin. Cancer Res. 16(2):755-763(2010)
Grando, S.A., et al. J. Invest. Dermatol. 105(6):774-781(1995)
Girard, N., et al. Clin. Cancer Res. 16(2):755-763(2010)
Grando, S.A., et al. J. Invest. Dermatol. 105(6):774-781(1995)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57480
NCBI Official Full Name
neuronal acetylcholine receptor subunit alpha-3 isoform 1
NCBI Official Synonym Full Names
cholinergic receptor, nicotinic, alpha 3 (neuronal)
NCBI Official Symbol
CHRNA3
NCBI Official Synonym Symbols
LNCR2; PAOD2; NACHRA3
NCBI Protein Information
neuronal acetylcholine receptor subunit alpha-3
UniProt Protein Name
Neuronal acetylcholine receptor subunit alpha-3
UniProt Gene Name
CHRNA3
UniProt Synonym Gene Names
NACHRA3
UniProt Entry Name
ACHA3_HUMAN
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Product Notes
The CHRNA3 chrna3 (Catalog #AAA284257) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 25-52. The CHRNA3 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's CHRNA3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CHRNA3 chrna3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHRNA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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