Rabbit CHUK Polyclonal Antibody | anti-CHUK antibody

CHUK Antibody (N-term)

Cat. Num. AAA289249

• Gene Names: CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Mouse)
• Applications: ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: CHUK, Antibody; CHUK Antibody (N-term); Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16; CHUK; IKKA; TCF16; anti-CHUK antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Chicken, Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CHUK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 84-112 amino acids from the N-terminal region of human CHUK.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 84-112
• Sequence Length: 745

• Applicable Applications for anti-CHUK antibody: ELISA, WB (Western Blot)
• Function: Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Negatively regulates the pathway by phosphorylating the scaffold protein TAXBP1 and thus promoting the assembly of the A20/TNFAIP3 ubiquitin-editing complex (composed of A20/TNFAIP3, TAX1BP1, and the E3 ligases ITCH and RNF11). Therefore, CHUK plays a key role in the negative feedback of NF-kappa-B canonical signaling to limit inflammatory gene activation. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa- B RelB-p52 complexes. In turn, these complexes regulate genes encoding molecules involved in B-cell survival and lymphoid organogenesis. Participates also in the negative feedback of the non -canonicalNF-kappa-B signaling pathway by phosphorylating and destabilizing MAP3K14/NIK. Within the nucleus, phosphorylates CREBBP and consequently increases both its transcriptional and histone acetyltransferase activities. Modulates chromatin accessibility at NF-kappa-B-responsive promoters by phosphorylating histones H3 at 'Ser-10' that are subsequently acetylated at 'Lys-14' by CREBBP. Additionally, phosphorylates the CREBBP-interacting protein NCOA3.
• Cellular Location: Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus
• Tissue Location: Widely expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(CHUK Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CHUK antibody detected the CHUK protein (arrow).)

Related Product Information for anti-CHUK antibody

This gene encodes a member of the serine/threonine protein
kinase family. The encoded protein, a component of a
cytokine-activated protein complex that is an inhibitor of the
essential transcription factor NF-kappa-B complex, phosphorylates
sites that trigger the degradation of the inhibitor via the
ubiquination pathway, thereby activating the transcription factor.

Product Categories/Family for anti-CHUK antibody

Cardiovascular; Cell Biology; Immunology; Signal Transduction

References

Lahtela, J., et al. N. Engl. J. Med. 363(17):1631-1637(2010)
Gouin, E., et al. Proc. Natl. Acad. Sci. U.S.A. 107(40):17333-17338(2010)
Cummins, E.P., et al. J. Immunol. 185(7):4439-4445(2010)
Li, T., et al. Nat. Immunol. 11(9):799-805(2010)
Rotman, Y., et al. Hepatology 52(3):894-903(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 62241001
• NCBI GeneID: 1147
• NCBI Accession #: NP_001269.3
• NCBI GenBank Nucleotide #: NM_001278.3
• UniProt Accession #: O15111; O14666; Q13132; Q5W0I4; Q92467
• Molecular Weight: 84640
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit alpha
• NCBI Official Synonym Full Names: conserved helix-loop-helix ubiquitous kinase
• NCBI Official Symbol: CHUK
• NCBI Official Synonym Symbols: IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit alpha
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit alpha
• UniProt Gene Name: CHUK
• UniProt Synonym Gene Names: IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16
• UniProt Entry Name: IKKA_HUMAN