FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of Raji cells using anti-CIITA antibody (AAA126151).Overlay histogram showing Raji cells stained with AAA126151 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CIITA Antibody (AAA126151, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit CIITA Polyclonal Antibody | anti-CIITA antibody
Anti-CIITA Antibody Picoband
Gene Names
CIITA; C2TA; NLRA; MHC2TA; CIITAIV
Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Functional Assay
Purity
Immunogen affinity purified.
Synonyms
CIITA, Antibody; Anti-CIITA Antibody Picoband; anti-CIITA antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen affinity purified.
Form/Format
Liquid
Each vial contains 50% glycerol, 0.9% NaCI, 0.2% Na2HPO4, 0.02% NaN3
Each vial contains 50% glycerol, 0.9% NaCI, 0.2% Na2HPO4, 0.02% NaN3
Concentration
0.5 mg/ml (varies by lot)
Applicable Applications for anti-CIITA antibody
FCM/FACS (Flow Cytometry)
Immunogen
E.coli-derived human CIITA recombinant protein (Position: Y34-A1038). Human CIITA shares 69.6% amino acid (aa) sequence identity with mouse CIITA
Preparation and Storage
Store at -20 degree C for one year from date of receipt. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of Raji cells using anti-CIITA antibody (AAA126151).Overlay histogram showing Raji cells stained with AAA126151 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CIITA Antibody (AAA126151, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of Raji cells using anti-CIITA antibody (AAA126151).Overlay histogram showing Raji cells stained with AAA126151 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CIITA Antibody (AAA126151, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of CIITA using anti-CIITA antibody (AAA126151).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: rat spleen tissue lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse spleen tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIITA antigen affinity purified polyclonal antibody (#AAA126151) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CIITA at approximately 123 kDa. The expected band size for CIITA is at 123 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of CIITA using anti-CIITA antibody (AAA126151).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: rat spleen tissue lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse spleen tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIITA antigen affinity purified polyclonal antibody (#AAA126151) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CIITA at approximately 123 kDa. The expected band size for CIITA is at 123 kDa.)
Related Product Information for anti-CIITA antibody
CIITA is a human gene which is mapped to 16p13. This gene encodes a protein with an acidic transcriptional activation domain, 4 LRRs (leucine-rich repeats) and a GTP binding domain. The protein is located in the nucleus and acts as a positive regulator of class II major histocompatibility complex gene transcription, and is referred to as the "master control factor" for the expression of these genes. Also, the protein binds GTP and uses GTP binding to facilitate its own transport into the nucleus. Once in the nucleus it does not bind DNA but rather uses an intrinsic acetyltransferase (AT) activity to act in a coactivator-like fashion. Mutations in this gene have been associated with bare lymphocyte syndrome type II (also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency), increased susceptibility to rheumatoid arthritis, multiple sclerosis, and possibly myocardial infarction. Several transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-CIITA antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
MHC class II transactivator
NCBI Official Synonym Full Names
class II, major histocompatibility complex, transactivator
NCBI Official Symbol
CIITA
NCBI Official Synonym Symbols
C2TA; NLRA; MHC2TA; CIITAIV
NCBI Protein Information
MHC class II transactivator; NLR family, acid domain containing; MHC class II transactivator type III; nucleotide-binding oligomerization domain, leucine rich repeat and acid domain containing
UniProt Protein Name
MHC class II transactivator
UniProt Gene Name
CIITA
UniProt Synonym Gene Names
MHC2TA; CIITA
UniProt Entry Name
C2TA_HUMAN
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Product Notes
The CIITA ciita (Catalog #AAA126151) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CIITA Antibody Picoband reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CIITA can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CIITA ciita for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CIITA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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