IHC (Immunohistochemisry)
(Anti-CISH antibody, AAA45192, IHC(P)IHC(P): Rat Kidney Tissue)
Rabbit CISH Polyclonal Antibody | anti-CISH antibody
Anti-CISH antibody
Gene Names
CISH; CIS; G18; SOCS; CIS-1; BACTS2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
CISH, Antibody; Anti-CISH antibody; Cytokine-inducible SH2-containing protein(CIS); cytokine inducible SH2-containing protein; BACTS2 antibody; cis 1 antibody; CIS antibody; CIS-1 antibody; CIS1 antibody; CISH antibody; CISH_HUMAN antibody; Cytokine inducible inhibitor of signaling type 1B antibody; Cytokine inducible SH2 containing protein antibody; Cytokine-inducible SH2-containing protein antibody; G18 antibody; Protein G18 antibody; SOCS antibody; Suppressor of cytokine signaling antibody; anti-CISH antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
258
Applicable Applications for anti-CISH antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human CISH (241-258aa LPLPRRMADYLRQYPFQL), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohistochemisry)
(Anti-CISH antibody, AAA45192, IHC(P)IHC(P): Rat Kidney Tissue)
IHC (Immunohistochemisry)
(Anti-CISH antibody, AAA45192, IHC(P)IHC(P): Rat Kidney Tissue)
IHC (Immunohiostchemistry)
(Anti-CISH antibody, AAA45192, IHC(P)IHC(P): Human Lung Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-CISH antibody, AAA45192, IHC(P)IHC(P): Human Lung Cancer Tissue)
WB (Western Blot)
(Anti-CISH antibody, AAA45192, Western blottingAll lanes: Anti CISH (AAA45192) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: A431 Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: MM231 Whole Cell Lysate at 40ugPredicted bind size: 29KDObserved bind size: 29KD)
WB (Western Blot)
(Anti-CISH antibody, AAA45192, Western blottingAll lanes: Anti CISH (AAA45192) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 50ugLane 2: Rat Kidney Tissue Lysate at 50ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: A431 Whole Cell Lysate at 40ugLane 5: SMMC Whole Cell Lysate at 40ugLane 6: HELA Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: MM231 Whole Cell Lysate at 40ugPredicted bind size: 29KDObserved bind size: 29KD)
Related Product Information for anti-CISH antibody
Description: Rabbit IgG polyclonal antibody for Cytokine-inducible SH2-containing protein(CISH) detection. Tested with WB, IHC-P, ICC in Human, Mouse, Rat.
Background: CISH(cytokine inducible SH2-containing protein),also called CIS, CIS-1, G18, SOCS, is an important negative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling(SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is mapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on chromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by lipopolysaccharide(LPS) or Cryptosporidium parvum exposure, and this upregulation involved downregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression of CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and enhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.
Background: CISH(cytokine inducible SH2-containing protein),also called CIS, CIS-1, G18, SOCS, is an important negative regulator for inflammatory signaling and belongs to the suppressors of cytokine signaling(SOCS) family. CIS family members are known to be cytokine-inducible negative regulators of cytokine signaling. CISH controls interleukin-2 signaling, and variations of CISH with certain SNPs are associated with susceptibility to bacteremia, tuberculosis and malaria. The human CISH gene is mapped to chromosome 3p21.3 by FISH. The mouse gene is tightly linked to the Gnai2 gene on chromosome 9, a region syntenic to human chromosome 3p21. CIS expression was upregulated by lipopolysaccharide(LPS) or Cryptosporidium parvum exposure, and this upregulation involved downregulation of MIR98 and LET7, which relieved MIR98- and LET7-mediated translational repression of CIS. Gain- and loss-of-function studies showed that CIS accelerated degradation of IKBA and enhanced NFKB activation in cholangiocytes in response to LPS stimulation or C parvum exposure.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,734 Da
NCBI Official Full Name
Cytokine-inducible SH2-containing protein
NCBI Official Synonym Full Names
cytokine inducible SH2-containing protein
NCBI Official Symbol
CISH
NCBI Official Synonym Symbols
CIS; G18; SOCS; CIS-1; BACTS2
NCBI Protein Information
cytokine-inducible SH2-containing protein; suppressor of cytokine signaling; cytokine-inducible inhibitor of signaling type 1B
UniProt Protein Name
Cytokine-inducible SH2-containing protein
UniProt Gene Name
CISH
UniProt Synonym Gene Names
G18; CIS; SOCS
UniProt Entry Name
CISH_HUMAN
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Product Notes
The CISH cish (Catalog #AAA45192) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CISH antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CISH can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CISH cish for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CISH, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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