Rabbit CKB Polyclonal Antibody | anti-CKB antibody

Phospho-CKB (Ser199) Antibody

Cat. Num. AAA329963

• Gene Names: CKB; BCK; B-CK; CKBB; HEL-211; HEL-S-29
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: CKB, Antibody; Phospho-CKB (Ser199) Antibody; B CK; B-CK; BB-CK; BCK; Brain creatine kinase; Ckb; CKBB; Creatine kinase B; Creatine kinase B chain; Creatine kinase B type; Creatine kinase B-type; Creatine Kinase BB Isoenzyme; Creatine kinase brain; Creatine kinase brain type; Creatine phosphokinase BB; Epididymis luminal protein 211; Epididymis secretory protein Li 29; HEL 211; HEL S 29; KCRB_HUMAN; anti-CKB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-CKB (Ser199) Antibody detects endogenous levels of CKB only when phosphorylated at S199.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CKB antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human CKB around the phosphorylation site of S199.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Subunit Structure: Dimer of identical or non-identical chains, which can be either B (brain type) or M (muscle type). With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.
• Similarity: Belongs to the ATP:guanido phosphotransferase family.
• Subcellular Location: Cytoplasm.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemistry)

(At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemisry)

(At 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from Jurkat, using Phospho-CKB(S199) Antibody.)

WB (Western Blot)

(Western blot analysis of extracts from Jurkat, using Phospho-CKB(Ser199) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Related Product Information for anti-CKB antibody

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

NCBI and Uniprot Product Information

• NCBI GI #: 125294
• NCBI GeneID: 1152
• UniProt Accession #: P12277; Q2LE07; Q6FG40; Q9UC66; A8K236; B2R5R4
• Molecular Weight: 381
• NCBI Official Full Name: Creatine kinase B-type
• NCBI Official Synonym Full Names: creatine kinase, brain
• NCBI Official Symbol: CKB
• NCBI Official Synonym Symbols: BCK; B-CK; CKBB; HEL-211; HEL-S-29
• NCBI Protein Information: creatine kinase B-type; creatine kinase-B; creatine kinase B chain; epididymis luminal protein 211; epididymis secretory protein Li 29
• UniProt Protein Name: Creatine kinase B-type
• UniProt Gene Name: CKB
• UniProt Synonym Gene Names: CKBB
• UniProt Entry Name: KCRB_HUMAN