WB (Western Blot)
(Detection of human CKI epsilon by western blot. Samples: Whole cell lysate (5, 15, and 50 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-CKI epsilon antibody AAA211775 (lot AAA211775-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human CKI epsilon Polyclonal Antibody | anti-CKIepsilon antibody
Rabbit anti-CKI epsilon Antibody, Affinity Purified
Gene Names
CSNK1E; HCKIE; CKIepsilon
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
CKI epsilon, Antibody; Rabbit anti-CKI epsilon Antibody, Affinity Purified; CKIe; HCKIE; casein kinase I isoform epsilon; CKIepsilon; CKI-epsilon; Casein kinase I isoform epsilon; casein kinase 1 epsilon; anti-CKIepsilon antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-CKIepsilon antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse, Chicken
Immunogen
Between 366 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human CKI epsilon by western blot. Samples: Whole cell lysate (5, 15, and 50 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-CKI epsilon antibody AAA211775 (lot AAA211775-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human CKI epsilon by western blot. Samples: Whole cell lysate (5, 15, and 50 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-CKI epsilon antibody AAA211775 (lot AAA211775-1) used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human CKI epsilon by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKI epsilon antibody AAA211775 (lot A302-0135A-1) used for IP at 10 ug/mg lysate. CKI epsilon was also immunoprecipitated by a rabbit antibody (BL7891) directed to an epitope that is common to CKI epsilon and CKI delta. CKI delta was immunoprecipitated and blotted using For blotting immunoprecipitated CKI epsilon, AAA211775 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human CKI epsilon by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CKI epsilon antibody AAA211775 (lot A302-0135A-1) used for IP at 10 ug/mg lysate. CKI epsilon was also immunoprecipitated by a rabbit antibody (BL7891) directed to an epitope that is common to CKI epsilon and CKI delta. CKI delta was immunoprecipitated and blotted using For blotting immunoprecipitated CKI epsilon, AAA211775 was used at 1.0 ug/ml. Detection: Chemiluminescence with exposure time of 3 seconds.)
Related Product Information for anti-CKIepsilon antibody
CKI epsilon is a member of the casein kinase 1 (CK1) serine/threonine kinase family of proteins. In mammals, there are seven distinct genes that represent the CK1 family: alpha, beta, gamma-1, gamma-2, gamma-3, delta, and epsilon. CKI epsilon phosphorylates a large number of proteins and is involved in pathways involving Wnt and AKT. CKI epsilon has been found to bind and phosphorylate the circadian protein, Period, and the Wnt signaling protein, Dsh. In the Wnt pathway, CKI epsilon activity is required for the full activation of Dsh and downstream signaling effects.
Product Categories/Family for anti-CKIepsilon antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,315 Da
NCBI Official Full Name
casein kinase I isoform epsilon
NCBI Official Synonym Full Names
casein kinase 1, epsilon
NCBI Official Symbol
CSNK1E
NCBI Official Synonym Symbols
HCKIE; CKIepsilon
NCBI Protein Information
casein kinase I isoform epsilon; CKIe; CKI-epsilon
UniProt Protein Name
Casein kinase I isoform epsilon
UniProt Gene Name
CSNK1E
UniProt Synonym Gene Names
CKI-epsilon; CKIe
UniProt Entry Name
KC1E_HUMAN
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Product Notes
The CKIepsilon csnk1e (Catalog #AAA211775) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-CKI epsilon Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CKI epsilon can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the CKIepsilon csnk1e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CKI epsilon, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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