Rabbit anti-Human CKMT1A Polyclonal Antibody | anti-CKMT1A antibody

Anti-CKMT1A Antibody, Rabbit Polyclonal

Cat. Num. AAA256225

• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: CKMT1A, Antibody; Anti-CKMT1A Antibody, Rabbit Polyclonal; CKMT1A Antibody, Rabbit PAb, Antigen Affinity Purified; creatine kinase, mitochondrial 1A; Anti-CKMT Antibody; Anti-CKMT1 Antibody; Anti-CKMT1A Antibody; anti-CKMT1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CKMT1A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-CKMT1A antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: E Coli-derived Human CKMT1A fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human CKMT1A fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(CKMT1A was immunoprecipitated using:Lane A:0.5 mg A431 Whole Cell Lysate4 uL anti-CKMT1A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-CKMT1A rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size :40 kDa)

WB (Western Blot)

(Anti-CKMT1A rabbit polyclonal antibody at 1:500 dilutionLane A: A-431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:47 kDaObserved band size:43 kDa)

Related Product Information for anti-CKMT1A antibody

CKMT1A belongs to the ATP:guanido phosphotransferase family. It contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. CKMT1A gene is one of two genes that encodes the ubiquitous mitochondrial creatine kinase (CKMT1). CKMT1 is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK, encoded by separate genes. CKMT1 occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric CKMT1.

References

Haas RC, et al. (1989) Isolation and characterization of the gene and cDNA encoding human mitochondrial creatine kinase. J Biol Chem. 264(5):2890-7.Stachowiak O, et al. (1998) Oligomeric state and membrane binding behaviour of creatine kinase isoenzymes: implications for cellular function and mitochondrial structure. Mol Cell Biochem. 184(1-2):141-51.Lipskaya TY. (2001) Mitochondrial creatine kinase: properties and function. Biochemistry Mosc. 66(10):1098-111.