IHC (Immunohiostchemistry)
(Anti- Claudin 2 antibody, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
anti-Human, Rat Claudin 2 Polyclonal Antibody | anti-CLDN2 antibody
Anti-Claudin 2 Antibody
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Claudin 2, Antibody; Anti-Claudin 2 Antibody; Claudin-2; Claudin2; CLD2_HUMAN; CLDN 2; CLDN2; OTTHUMP00000023793; SP 82; SP82; claudin 2; anti-CLDN2 antibody
Reactivity
Human, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
230
Applicable Applications for anti-CLDN2 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human Claudin 2 recombinant protein (Position: A38-V230). Human Claudin 2 shares 92% amino acid (aa) sequence identity with mouse Claudin 2.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti- Claudin 2 antibody, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti- Claudin 2 antibody, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
WB (Western Blot)
(Anti- Claudin 2 antibody, Western blottingAll lanes: Anti Claudin 2 at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugPredicted bind size: 25KDObserved bind size: 25KD)
WB (Western Blot)
(Anti- Claudin 2 antibody, Western blottingAll lanes: Anti Claudin 2 at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 50ugLane 2: Rat Liver Tissue Lysate at 50ugPredicted bind size: 25KDObserved bind size: 25KD)
Related Product Information for anti-CLDN2 antibody
Description: Rabbit IgG polyclonal antibody for Claudin-2(CLDN2) detection. Tested with WB, IHC-P in Human;Rat.
Background: Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter.
Background: Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. By genomic sequence analysis, this gene is mapped to chromosome Xq22.3-q23. This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. By expression in a human intestinal epithelial cell line, it was determined that the intestine-specific homeodomain proteins CDX1 and CDX2 activated a reporter plasmid driven by the CLDN2 promoter.
References
1. Morita K, Furuse M, Fujimoto K, Tsukita S (Mar 1999). "Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands". Proc Natl Acad Sci U S A 96 (2): 511-6. 2. Sakaguchi, T., Gu, X., Golden, H. M., Suh, E., Rhoads, D. B., Reinecker, H.-C. Cloning of the human claudin-2 5-prime-flanking region revealed a TATA-less promoter with conserved binding sites in mouse and human for caudal-related homeodomain proteins and hepatocyte nuclear factor-1-alpha. J. Biol. Chem. 277: 21361-21370, 2002. 3. Muto, S.; Hata, M.; Taniguchi, J.; Tsuruoka, S.; Moriwaki, K.; Saitou, M.; Furuse, K.; Sasaki, H.; Fujimura, A.; Imai, M.; Kusano, E.; Tsukita, S.; Furuse, M. (2010). "Claudin-2-deficient mice are defective in the leaky and cation-selective paracellular permeability properties of renal proximal tubules". Proceedings of the National Academy of Sciences 107 (17): 8011-8016.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,549 Da
NCBI Official Full Name
claudin-2
NCBI Official Synonym Full Names
claudin 2
NCBI Official Symbol
CLDN2
NCBI Protein Information
claudin-2
UniProt Protein Name
Claudin-2
UniProt Gene Name
CLDN2
UniProt Entry Name
CLD2_HUMAN
Similar Products
Product Notes
The CLDN2 cldn2 (Catalog #AAA46213) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Claudin 2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Claudin 2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CLDN2 cldn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Claudin 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.