Application Data
(Overlay histogram showing Hela cellsstained with (AAA290894)(green line). The cellswere fixed with 2% paraformaldehyde (10min) and then permeabilized with 90%methanol for 10 min. The cells were thenicubated in 2% bovine serum albumin to block non-specific protein-protein interactionsfollowed by the antibody (AAA290894, 1:25dilution) for 60 min at 37ºC. The secondaryantibody used was Goat-Anti-Rabbit IgG,DyLight® 488 Conjugated HighlyCross-Adsorbed(1583138) at 1/200 dilutionfor 40 min at 37ºC. Isotype control antibody(blue line) was rabbit IgG1 (1?g/1x10^6cells) used under the same conditions.Acquisition of >10, 000 events wasperformed.)
Rabbit anti-Human, Mouse CLCN1 Polyclonal Antibody | anti-CLCN1 antibody
CLCN1 Antibody (N-Term)
Gene Names
CLCN1; CLC1
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
CLCN1, Antibody; CLCN1 Antibody (N-Term); Purified Rabbit Polyclonal Antibody (Pab); Chloride channel protein 1; ClC-1; Chloride channel protein; skeletal muscle; CLCN1; CLC1; anti-CLCN1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Ig
Specificity
This CLCN1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 32-66 amino acids from the human region of human CLCN1.
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
988
Applicable Applications for anti-CLCN1 antibody
ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry)
Tissue Location
Predominantly expressed in skeletal muscles.
Cellular Location
Cell membrane; Multi-pass membrane protein
Function
Voltage-gated chloride channel (PubMed: 8112288, PubMed: , PubMed: ). Plays an important role in membrane repolarization in skeletal muscle cells after muscle contraction. The CLC channel family contains both chloride channels and proton-coupled an ion transporters that exchange chloride or another anion for protons (Probable). The absence of conserved gating glutamate residues is typical for family members that function as channels (Probable).
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Application Data
(Overlay histogram showing Hela cellsstained with (AAA290894)(green line). The cellswere fixed with 2% paraformaldehyde (10min) and then permeabilized with 90%methanol for 10 min. The cells were thenicubated in 2% bovine serum albumin to block non-specific protein-protein interactionsfollowed by the antibody (AAA290894, 1:25dilution) for 60 min at 37ºC. The secondaryantibody used was Goat-Anti-Rabbit IgG,DyLight® 488 Conjugated HighlyCross-Adsorbed(1583138) at 1/200 dilutionfor 40 min at 37ºC. Isotype control antibody(blue line) was rabbit IgG1 (1?g/1x10^6cells) used under the same conditions.Acquisition of >10, 000 events wasperformed.)
Application Data
(Overlay histogram showing Hela cellsstained with (AAA290894)(green line). The cellswere fixed with 2% paraformaldehyde (10min) and then permeabilized with 90%methanol for 10 min. The cells were thenicubated in 2% bovine serum albumin to block non-specific protein-protein interactionsfollowed by the antibody (AAA290894, 1:25dilution) for 60 min at 37ºC. The secondaryantibody used was Goat-Anti-Rabbit IgG,DyLight® 488 Conjugated HighlyCross-Adsorbed(1583138) at 1/200 dilutionfor 40 min at 37ºC. Isotype control antibody(blue line) was rabbit IgG1 (1?g/1x10^6cells) used under the same conditions.Acquisition of >10, 000 events wasperformed.)
WB (Western Blot)
(All lanes : Anti-CLCN1 Antibody (N-Term) at 1:2000 dilutionLane 1: Human skeletal muslce lysateLane 2: Mouse skeletal muscle lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 109 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-CLCN1 Antibody (N-Term) at 1:2000 dilutionLane 1: Human skeletal muslce lysateLane 2: Mouse skeletal muscle lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 109 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-CLCN1 antibody
Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport.
Product Categories/Family for anti-CLCN1 antibody
References
Steinmeyer K.,et al.EMBO J. 13:737-743(1994). Scherer S.W.,et al.Science 300:767-772(2003). Koch M.C.,et al.Science 257:797-800(1992). George A.L. Jr.,et al.Nat. Genet. 3:305-310(1993). Lorenz C.,et al.Hum. Mol. Genet. 3:941-946(1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
108626
NCBI Official Full Name
chloride channel protein 1
NCBI Official Synonym Full Names
chloride voltage-gated channel 1
NCBI Official Symbol
CLCN1
NCBI Official Synonym Symbols
CLC1
NCBI Protein Information
chloride channel protein 1
UniProt Protein Name
Chloride channel protein 1
UniProt Gene Name
CLCN1
UniProt Synonym Gene Names
CLC1; ClC-1
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Product Notes
The CLCN1 clcn1 (Catalog #AAA290894) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLCN1 Antibody (N-Term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CLCN1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CLCN1 clcn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLCN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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